초록 |
Microalgae-derived biomass is currently considered a sustainable feedstock for making biofuels. The photoautotrophic cultivation using flue gas has been continuously investigated to improve the economic feasibility of microalgae processes. The utilization of waste CO2 is advantageous in reducing carbon footprints and the cost of carbon sources. If CO2 supply is suddenly halted during cultivation, biomass productivity is greatly reduced. To prevent this condition, urea and KH2PO4 are added to the medium. Urea and KH2PO4 were present in the medium in the form of CO2/NH4+ and K+/H2PO4−, respectively, preventing cell growth inhibition. The culture with urea and KH2PO4 supplementation exhibited 7.28-10.02-fold higher biomass and lipid productivity, compared to the culture with ambient CO2 supply due to the maintenance of a stable pH and dissolved inorganic carbon in the medium. Consequently, the microalgae were grown successfully without cell death. |