초록 |
SI-ARGET ATRP of monomers containing 2-aminoethyl methacrylate hydrochloride and oligo(ethylene glycol) methacrylate has performed on a gold presenting ATRP initiators, and subsequently the poly(AMA-r-OEGMA) films was coupled with biotin containing N-hydroxysuccinimide esters (Biotin-NHS), resulting in biotinylated, poly(AMA-r-OEGMA) films. The microcontact printing of Biotin-NHS via PDMS stamping and subsequent incubation of the patterned films in rhodamine-conjugated streptavidin solution was performed on biotinylated, poly(AMA-r-OEGMA) films and the micropatterns of proteins were observed via confocal microscopy. |