| 초록 |
Tumor-specific drug and gene delivery is critically important for the successful application of anticancer agents. Poly(D,L-lactic-co-glycolic acid) has been widely used for tumor-targeted drug delivery due to its biodegradability and biocompatibility. However, it is very challeging to encapsulate hydrophilic macromolecules with a high loading efficiency. In this study, we investigate the incorporation of lipid-siRNA complexes with PLGA nanoparticles for targerted siRNA delivery. DOTAP is complexed with siRNA in DMSO and encapsulated into surface modified PLGA using the simple nanoprecipitation. The loading efficiency of siRNA into PLGA nanoparticles is about 77.6 %. The hydrodynamic diameter and zeta potential of DOTAP/siRNA-loaded PLGA nanoparticles are 224.0 ± 3.2 nm and - 3.2 ± 2.6 mV, respectively. This study suggests that the lipid-siRNA complexation can be used to effectively increase the encapsulation efficiency of siRNA within a hydrophobic solid polymer nanoparticles. |
