| 학회 |
한국공업화학회 |
| 학술대회 |
2007년 가을 (11/02 ~ 11/03, 한경대학교) |
| 권호 |
11권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Discovery of novel NRPS product from Streptomyces peucetius by genome mining and mass spectrometry |
| 초록 |
One of the putative NRPS (Nonribosomal Peptide Synthase) gene clusters of Streptomyces Peucetius is selected and its domain architecture is predicted by homology search within NRPS database. The product of this gene cluster is predicted as a siderophore that is ferric-specific ligand to scavenge and transport iron into the cells. Isolated peptides are confirmed to be a hydroxamate group siderophore by Universal CAS assay and Csaky test. Its molecular formula is predicted by exact mass measurement with LTQ-Orbitrap and further analysis of MS/MS fragmentation patterns allowed proposal of hypothetical structure. Based on proposed structure, NMR analysis is in progress. |
| 저자 |
장동숙, 주황수, 김평일, 김병기
|
| 소속 |
서울대 |
| 키워드 |
Nonribosomal peptide; Streptomyces; Siderophore; Mass spectrometry
|
| E-Mail |
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