| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
37권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
One-Pot Encapsulation of Nile Red via Protein-Polymer Conjugation |
| 초록 |
Polymeric micelles have emerged as a novel promising colloidal carrier for the targeting of poorly water soluble drugs and nanoparticles. Herein we describe a one pot method for encapsulation of Nile Red into micellar aggregates using nickel complexed (nitrilotriacetic acid)-end-functionalized polystyrene (Ni-NTA-PS) and histidine-tagged green fluorescent protein (His-tagged GFP) in water/DMF (DMF 4 vol %). When Ni-NTA-PS and Nile Red dissolved in DMF was added slowly into PBS buffer containing His-tagged GFP, GFP coated micellar aggregates were formed and Nile Reds were encapsulated inside the micellar aggregates at once. The formation of micellar aggregate was confirmed by transmission electron microscopy (TEM) measurement and dynamic light scattering (DLS) study. Encapsulation of Nile Red was confirmed by photoluminescence (PL) spectroscopy and fluorescent microscopy. |
| 저자 |
이채연, Mohammad Abdul Kadir, 백현종
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| 소속 |
부산대 |
| 키워드 |
bioconjugation; self-assembly; encapsulation
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| E-Mail |
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