미녹시딜을 내포한 용융형 고분자 마이크로 니들의 길이에 따른 특성 및 발모 효능 관찰

한미리; 김은영; 정용현; 김명석; 박정환; Mee-Ree Han; Eun-Young Kim; Yong-Hyun Jung; Myoung-Seok Kim; Jung-Hwan Park

Polymer(Korea), Vol.37, No.3, 393-398, May, 2013

Characteristics and Hair Growth Efficacy of Water Dissolving Micro-needles Containing Minoxidil Regarding Length of Micro-needles

한미리, 김은영¹, 정용현¹, 김명석¹, 박정환^{2, †}

중앙대학교 융합의약학과
¹B2Y Korea Ltd.
²가천대학교 바이오나노학과

Mee-Ree Han, Eun-Young Kim¹, Yong-Hyun Jung¹, Myoung-Seok Kim¹, and Jung-Hwan Park^{2, †}

School of Medicine, Chung Ang University, Dongjak-gu, Seoul 156-861, Korea
¹B2Y Korea Ltd., Cheonan-si, Chungcheongnam-do 331-833, Korea
²College of BioNano Technology and Gachon BioNano Research Institute, Gachon University, Seongnam-si, Gyeonggi-do 461-701, Korea

E-mail:

초록

본 실험에선 미녹시딜을 함유한 수용성 고분자 젤로부터 solvent casting 방법으로 250과 750 μm 두 가지 길이의 미녹시딜이 함유된 용융형 고분자 마이크로 니들(micro-needle)을 제작하였다. 제작된 미녹시딜을 함유한 용융형 고분자 마이크로 니들은 기계적 강도가 입증된 고분자 니들과 동일한 형태 및 기계적 강도를 나타냈으며, 성공적으로 피부를 투과함을 확인하였다. 제조된 고분자 마이크로 니들의 경우 피부 적용 후 최소 30분 이상은 부착되어야 전체 니들의 50% (v/v) 이상이 피부 내로 용융되었으며, 니들 적용부위에서 서서히 약물의 방출 및 확산을 확인할 수 있었다. 또한 C57BL/6 동물 모델을 통해 미녹시딜이 함유된 250, 750 μm 길이의 고분자 마이크로 니들이무처리군 혹은 미녹시딜 처리군에 비해 발모에 긍정적인 영향을 나타냄을 확인할 수 있었다.

Two types of polymeric micro-needles with 250 and 750 μm lengths were prepared by a micromolding process. The geometry of dissolving micro-needles with minoxidil was investigated and the mechanical property of polymer micro-needles was also evaluated. Water dissolving micro-needles containing minoxidil were successfully inserted into skin and delivered minoxidil to the skin layer after 30 min of insertion. The observed hair growth was less when the minoxidil treatment was not applied or used without the micro-needles than that the minoxidil was applied by micro-needles. Histologically, these hair follicles became bigger, and the hair papillae were totally encircled by the hair bulbs. In this feasibility test, we show the successful delivery of minoxidil, and effective treatment of hair loss can be possible by using dissolving micro-needles with minoxidil.

Keywords:water dissolving polymer;micro-needle;microstructure;micromolding;hair growth.

[References]

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Wasserman D, Guzman-Sanchez DA, Scott K, McMichael A, Int. J. Dermatol., 46, 121 (2007)
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Waiz M, Saleh AZ, Hayani R, Jubory SO, J. Cosmet.Laser Ther.,, 8, 27 (2006)
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Nelson DA, Spielvogel RL, Int. J. Dermatol., 24, 606 (2007)
Italia JL, Bhardwaj V, Kumar MR, Drug Discov. Today., 11, 846 (2006)
Sung YK, Hwang SY, Cha SY, Kim SR, Park SY, Kim MK, Kim JC, J. Dermatol. Sci., 41, 150 (2006)
Fischer T, Hipler U, Elsner P, Int. J. Dermatol., 46, 27 (2007)
Otberg N, Teichmann A, Rasuljev U, Sinkgraven R, Sterry W, Lademann J, Skin Pharmacol. Phys., 20, 195 (2007)
Olsen EA, Messenger AG, Shapiro J, Bergfeld WF, Hordinsky MK, Roberts JL, Stough D, Washenik K, Whiting DA, J. Am. Acad. Dermatol., 52, 301 (2005)
Mura S, Pirot F, Manconi M, Falson F, Fadda AM, J. Drug Target., 15, 101 (2007)
Lee YB, Eun YS, Lee JH, Cheon MS, Park YG, Cho BK, Park HJ, The Journal of Dermatology., 40, 81 (2013)
Park JH, Choi SO, Seo S, Choy YB, Prausnitz MR, Eur. J. Pharm. Biopharm., 76, 282 (2010)
Lee JW, Han MR, Park JH, Journal of Drug Targeting., DOI: 10.3109/1061186X.2012. 741136 (2013)
Kolli CS, Banga AK, Pharm. Res., 25, 104 (2008)
Li G, Badkar A, Nema S, Kolli CS, Banga AK, Int. J.Pharmaceut., 368, 109 (2009)
Ito Y, Murakami A, Maeda T, Sugioka N, Takada K, Int.J. Pharmaceut., 349, 124 (2008)
Lee JW, Choi SO, Felner EI, Prausnitz MR, Small., 7, 531 (2011)
Matsuo K, Hirobe S, Yokota Y, Ayabe Y, Seto M, Quan YS, Kamiyama F, Tougan T, Horii T, Mukai Y, J. Control.Release., 160, 495 (2012)
Kommareddy S, Baudner BC, Oh S, Kwon S, Singh M, O'hagan DT, J. Pharm. Sci., 101, 1021 (2012)
Lee JW, Park JH, Prausnitz MR, Biomaterials., 29, 2113 (2008)
Kim MY, Jung B, Park JH, Biomaterials., 33, 668 (2011)

[Referenced By]

[1] McElwee K, Tobin D, Bystryn JC, King L, Sundberg J, Exp. Dermatol., 8, 371 (2007)

[2] Wasserman D, Guzman-Sanchez DA, Scott K, McMichael A, Int. J. Dermatol., 46, 121 (2007)

[3] Gilhar A, Kalish RS, Autoimmun. Rev., 5, 64 (2006)

[4] Al-Mutairi N, Dermatol. Surg., 33, 1483 (2007)

[5] Waiz M, Saleh AZ, Hayani R, Jubory SO, J. Cosmet.Laser Ther.,, 8, 27 (2006)

[6] Olsen EA, Dunlap FE, Funicella T, Koperski JA, Swinehart JM, Tschen EH, Trancik RJ, J. Am. Acad. Dermatol., 47, 377 (2002)

[7] Lucky AW, D. J. Piacquadio DJ, Ditre CM, Dunlap F, Kantor I, Pandya AG, Savin RC, Tharp MD, J. Am. Acad. Dermatol., 50, 541 (2004)

[8] Nelson DA, Spielvogel RL, Int. J. Dermatol., 24, 606 (2007)

[9] Italia JL, Bhardwaj V, Kumar MR, Drug Discov. Today., 11, 846 (2006)

[10] Sung YK, Hwang SY, Cha SY, Kim SR, Park SY, Kim MK, Kim JC, J. Dermatol. Sci., 41, 150 (2006)

[11] Fischer T, Hipler U, Elsner P, Int. J. Dermatol., 46, 27 (2007)

[12] Otberg N, Teichmann A, Rasuljev U, Sinkgraven R, Sterry W, Lademann J, Skin Pharmacol. Phys., 20, 195 (2007)

[13] Olsen EA, Messenger AG, Shapiro J, Bergfeld WF, Hordinsky MK, Roberts JL, Stough D, Washenik K, Whiting DA, J. Am. Acad. Dermatol., 52, 301 (2005)

[14] Mura S, Pirot F, Manconi M, Falson F, Fadda AM, J. Drug Target., 15, 101 (2007)

[15] Lee YB, Eun YS, Lee JH, Cheon MS, Park YG, Cho BK, Park HJ, The Journal of Dermatology., 40, 81 (2013)

[16] Park JH, Choi SO, Seo S, Choy YB, Prausnitz MR, Eur. J. Pharm. Biopharm., 76, 282 (2010)

[17] Lee JW, Han MR, Park JH, Journal of Drug Targeting., DOI: 10.3109/1061186X.2012. 741136 (2013)

[18] Kolli CS, Banga AK, Pharm. Res., 25, 104 (2008)

[19] Li G, Badkar A, Nema S, Kolli CS, Banga AK, Int. J.Pharmaceut., 368, 109 (2009)

[20] Ito Y, Murakami A, Maeda T, Sugioka N, Takada K, Int.J. Pharmaceut., 349, 124 (2008)

[21] Lee JW, Choi SO, Felner EI, Prausnitz MR, Small., 7, 531 (2011)

[22] Matsuo K, Hirobe S, Yokota Y, Ayabe Y, Seto M, Quan YS, Kamiyama F, Tougan T, Horii T, Mukai Y, J. Control.Release., 160, 495 (2012)

[23] Kommareddy S, Baudner BC, Oh S, Kwon S, Singh M, O'hagan DT, J. Pharm. Sci., 101, 1021 (2012)

[24] Lee JW, Park JH, Prausnitz MR, Biomaterials., 29, 2113 (2008)

[25] Kim MY, Jung B, Park JH, Biomaterials., 33, 668 (2011)