화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.38, No.3, 278-285, May, 2014
Export Citation
탈미네랄골분이 첨가된 알지네이트 스펀지에서 조직공학적 연골 재건
Tissue Engineered Catilage Reconstruction with Alginate Sponge Containing Demineralized Bone Particles
김혜민, 박진영, 김은영, 송정은, 권순용1, 정진화1, †, 강길선†
  • 전북대학교 BIN 융합공학과
  • 1가톨릭대학교 의과대학 여의도성모병원 정형외과
Hye Min Kim, Jin Young Park, Eun Young Kim, Jeong Eun Song, Soon Yong Kwon1, Jin Wha Chung1, †, and Gilson Khang†
  • Dept. of BIN Fusion Tech, Polymer Fusion Res Center & Dept. of PolymerNano Sci Tech, Chonbuk National University, 567 Deokjin, Jeonju, Chonbuk 561-756, Korea
  • 1Dept. of Orthopedic Surgery, Yeouido St. Mary’s Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-896, Korea
E-mail:,
초록
알지네이트와 탈미네랄골분(DBP) 하이브리드 스펀지는 각각 DBP가 0, 10, 20, 40 및 80% 첨가된 스펀지로, 연골 재건을 위해 제작되었다. 세포의 증식은 MTT를 통해 관찰했으며, 형태학적, 조직학적, 생물학적 관찰 그리고 RT-PCR을 1, 2, 및 3 주에 실시하였다. 세포생존율에 있어서 20% DBP/알지네이트 스펀지에서 다른 스펀지보다도 가장 좋은 결과를 보였으며, 광학전자현미경 관찰에서 또한 20% 스펀지에서 가장 좋은 세포부착률과 증식률을 확인하였다. 마지막으로, 조직학적 평가에서 20% 스펀지에서 연골세포의 표현형이 가장 잘 유지됨과 동시에 산성뮤코 다당류와 제2형 콜라겐이 잘 형성된 것을 확인하였다. 이로써 DBP/알지네이트 하이브리드 스펀지는 연골 조직공학을 위한 지지체로써 활용될 수 있을 것이다.
Demineralized bone particles (DBP) and alginate hybrid sponges were fabricated at 10, 20, 40 and 80% DBP/alginate hybrid ratios for seeding chondrocyte. Cell proliferation was measured via MTT assay. Morphological observation, histology, biological assay and RT-PCR were performed at each time point 1, 2 and 3 weeks. The cell viability was better in 20% DBP/alginate sponges than in other sponges. SEM results showed that more attached and more proliferated cells in the 20% DBP/alginate sponges with the lapse of time. Finally, histochemical assay results showed that the phenotype of chondrocyte was well maintained and both acidic mucopolysaccharide and type II collagen was well formed at 20% sponges. This study suggested that DBP/alginate sponge may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage.
Keywords:alginate sponge;DBP (demineralized bone particle);chondrocyte;reconstruction.
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