1-Ferrocenylethanol and 1-ferrocenyl-2,2,2-trifluoroethanol were resolved with transesterification by lipases to the optically active (R)-esters and (S)-alcohols. Enzymatic hydrolysis of the racemic esters of 1-ferrocenylethanol using lipases yielded (R)-alcohol and (S)-esters. Enantioselective hydrolysis of racemic acetate or butanoate of 1-ferrocenyl-2,2,2-trifluoroethanol yielded the (S)-alcohol and (R)-esters. Enzymatic hydrolysis of (+/-)-acetate of 1-ferrocenyl-2, 2,2-trifluoroethanol yielded (R)-alcohol and (S)-acetate, but the equivalent (+/-)-butanoate was not hydrolyzed.