화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.26, No.4, 421-426, August, 2015
DOI10.14478/ace.2015.1042  Export Citation
나노기포 수소수에서 배양한 Arthrospira platensis 특성 확인
Characterization of Arthrospira platensis Cultured in Nano-bubble Hydrogen Water
서지혜1, 최수정1, 이상훈2, 3, 이재화1, †
  • 1신라대학교 의생명과학대학 제약공학
  • 2한국식품연구원 기능성식품연구본부
  • 3과학기술연합대학원대학교 식품생명공학
Ji-Hye Seo1, Soo-Jeong Choi1, Sang-Hoon Lee2, 3, and Jae-Hwa Lee1, †
  • 1Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University, Busan 617-736, Korea
  • 2Division of Functional Food Research, Korea Food Research Institute, Seongnam 463-746, Republic of Korea
  • 3Department of Food Biotechnology, University of Science and Technology, Daejeon 305-350, Republic of Korea
E-mail:
초록
미세조류 Arthrospira platensis는 단백질 함량이 높고, 불포화 지방산 등을 다량함유하고 있어 건강보조식품 및 다양한 분야에서 활용되고 있다. 또한 phycocyanin, myxoxanthophyll, zeaxanthin 등의 색소를 함유하고 있어서 항산화물질, 식품 첨가물로도 이용되고 있다. 나노기포 수소수는 수소 기체를 나노기포 상태로 물속에 주입하여 포화용해도 이상으로 용해시킨 것이다. 이런 나노기포 수소수는 항산화능이 높고, 항암효과가 있는 것으로 알려져 있다. Arthrospira platensis를 일반 증류수 배지와 수소수로 제조한 배지에서 배양한 후 특성을 확인하였다. 배양 결과, 세포 성장 및 광합성으로 인한 색소인 chlorophyll과 carotenoid의 함량은 수소수 배지에서 배양하였을 경우 대조군에 비해 15% 정도 증가한 것으로 나타난다. 그리고 phycocyanin 역시 7% 정도 증가하였다. 하지만, 지질함량은 수소수 배지 배양과 일반 배지배양 간에 큰 차이가 없는 것을 확인하였다. 항산화물질의 함량을 확인하기 위해 flavonoid 및 polyphenol의 함량을 측정하였다. Flavonoid는 수소수 배지에서 배양하였을 경우 대조군에 비해 70% 이상 증가함을 보이고 있다. 하지만 polyphenol은 대조군과 유사한 함량을 보이고 있다.
Arthrospira platensis (A. platensis) has been used in various fields including dietary supplements as it contains a high protein content and large amounts of unsaturated fatty acids. In addition, it has some pigments such as phycocyanin, myxoxanthophyll and zeaxanthin and thus has been used as a food additive and antioxidant substance. Nano-bubble hydrogen is to dissolve more than the saturation solubility in water by injecting the hydrogen gas in the nano-bubble hydrogen water. The nano-bubbles are known to possess higher antioxidant properties in addition to anticancer effects. In this paper, Arthrospira platensis was cultured in both a normal medium with distilled water and nano-bubble hydrogen water medium and their properties were compared. The cell growth and the content of chlorophyll and carotenoid in the nano-bubble hydrogen water was 15% higher than that of the control. The level of phycocyanin in nano-bubble hydrogen water was also 7% higher than that of the control. However, there were little differences in the lipid content between the nano-bubble and control. To determine the content of the antioxidants, the level of flavonoid and polyphenol were measured. The level of flavonoid in nano-bubble hydrogen water was found to be more than 70% increased when comparing to that of the control, while the level of polyphenol was similar to each other.
Keywords:Arthrospira platensis;nano-bubble hydrogen water;carotenoid;chlorophyll;antioxidant
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