오리발 유래 콜라겐/락타이드 글리콜라이드 공중합체 지지체에서의 골분화능 효과

국현; 김혜민; 김수민; 김은영; 송정은; 권순용; 서동삼; 박찬흠; 강길선; Hyeon Kuk; Hye Min Kim; Soo Min Kim; Eun Young Kim; Jeong Eun Song; Soon Yong Kwon; Dong Sam Suh; Chan Hum Park; Gilson Khang

Polymer(Korea), Vol.39, No.6, 846-851, November, 2015

DOI10.7317/pk.2015.39.6.846 Export Citation

오리발 유래 콜라겐/락타이드 글리콜라이드 공중합체 지지체에서의 골분화능 효과

Osteogenic Effect of Hybrid Scaffolds Composed of Duck Feet Collagen and PLGA

국현, 김혜민, 김수민, 김은영, 송정은 , 권순용 ¹, 서동삼², 박찬흠 ³, 강길선 ^†

전북대학교 BIN 융합공학과, 고분자나노공학과, 고분자 BIN융합 연구소
¹가톨릭대학교 의과대학 여의도성모병원 정형외과
²세원셀론텍
³한림대학교 의과대학 춘천성심병원 이비인후과

Hyeon Kuk, Hye Min Kim, Soo Min Kim, Eun Young Kim, Jeong Eun Song , Soon Yong Kwon¹, Dong Sam Suh², Chan Hum Park³, and Gilson Khang^†

Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University, 567 Baekje Daero, Deokjin, Jeonju, Chonbuk 561-756, Korea
¹Dept. of Orthepedic Surgery, Yeouido St. Mary’s Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-896, Korea
²Sewon Cellontech, 801, Wooyoung techno center, 273-15 Seongsu, 2ga 3-dong, Seongdong gu, Seoul 133-831, Korea
³Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Korea

E-mail:

초록

오리발 콜라겐은 최근에 조직공학에서 안정하고 안전한 재료로 사용되어 왔다. 본 연구에서는 PLGA 용액에 오리발에서 추출한 콜라겐을 비율별로 첨가하여 지지체를 준비하였다. DC/PLGA 지지체에 골수유래줄기세포를 배양하고 골분화능 및 골형성능을 체계적으로 비교하였다. 세포파종 후 초기 7day에서의 골분화는 DC함량에 비례하여 80%의 비율까지 넣은 PLGA 지지체에서 상당한 증가를 보여주었다. 하지만, DC/PLGA 지지체에서 ALP 활성도는 첫 주 이후, 점차적으로 감소하였고 14일 째에 기저수준에 도달하였다. 이 결과는 DC/PLGA 공중합체 지지체가 초기 뼈 형성과 분화에 도움을 주고 긍정적인 영향을 미친다는 것을 나타낸다.

Duck feet collagen (DC) has recently been used as a stable and safe source for tissue engineering. In this study, we have prepared a series of collagen scaffolds by mixing PLGA solutions with different percentages of collagen extracted from duck feet. Bone marow mesenchymal stem cells (BMSC) were cultured on the prepared DC/PLGA scaffolds, and their osteogenic effect and bone formation were compared systemically. At 7 days after seeding, osteogenisis was significantly increased on PLGA scaffolds containing upto 80% of DC. However, the alkaline phosphatase activity of DC/PLGA scaffolds decreased gradually after the first week and reached to the basal line after 14 days. This result indicates that DC/PLGA hybrid scaffolds are helpful for initial bone formation and differentiation, and have a positive effect on them.

Keywords:PLGA scaffold;collagen;ducks collagen;BMSC;bone regeneration;bone reconstruction

[References]

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Brinckmann J, Top. Curr. Chem., 247, 1 (2005)
Aszodi A, Legate KR, Nakchbandi I, Fassler R, Annu. Rev. Cell Dev. Biol., 22, 591 (2006)
Miyagi Y, Chiu LLY, Cimini M, Weisel RD, Radisic M, Weisel RD, Radisic M, Li RK, Biomaterials, 32, 1280 (2011)
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Rudnick A, J. Wound Care, 15, 402 (2006)
Parkman PD, Dev. Biol. Stand., 88, 5 (1996)
Horaud F, Dev. Biol. Stand., 75, 3 (1991)
Celis P, Silvester G, Dev. Biol. Stand., 118, 3 (2004)
Chen S, Hirota N, Okuda M, Takeguchi M, Kobayashi H, Hanagataa N, Ikom T, Acta Biomater., 7, 644 (2011)
Duan R, Zhang J, Du X, Yao X, Konno K, Food Chem., 112, 702 (2009)
Woo JW, Yu SJ, Cho SM, Lee YB, Kim SB, Food Hydrocolloids, 22, 879 (2008)
Arias-Moscoso JL, Soto-Valdez H, Plascencia-Jatomea M, Reyna-Luz Vidal-Quintanar, Rouzaud-Sndez O, Ezquerra-Brauer JM, Polym. Int., 60, 924 (2011)
Nishimoto S, Goto Y, Morishige H, Shiraishi R, Doi M, Akiyama K, Sugahara T, Biosci. Biotechnol. Biochem., 72, 2806 (2008)
Lee K, Park HY, Kim YK, Park JI, Yoon HD, J. Korean Soc. Appl. Biol. Chem., 52, 221 (2009)
Saiga A, Iwai K, Hayakawa T, Takahata Y, Kitamura S, Nishimura T, Morimatsu F, J. Agric. Food Chem., 56, 9586 (2008)
Pati F, Adhikari B, Dhara S, Bioresour. Technol., 101(10), 3737 (2010)
Ko HA, Kim HM, Ha HJ, Kim AR, Jang JE, Kim KH, Lee JH, Song JE, Yoon H, Lee OJ, Lee D, Park CH, Khang G, Inter. J. Tissue Regen., 3, 103 (2012)
Kim HM, Park JY, Kim EY, Song JE, Kwon SY, Chung JW, Khang G, Polym.(Korea), 38(3), 278 (2014)
Kim H, Kim HM, Jang JE, Kim CM, Kim EY, Lee D, Khang G, Int. J. Stem Cells, 6, 67 (2013)
Belbachir K, Noreen R, Gouspillou G, Petibois C, Anal. Bioanal. Chem., 395, 829 (2009)
Han DH, Kang HJ, An JJ, Jo HK, Yoo HR, Seol IC, Kim YS, Korean J. Orient. Physiol. Pathol., 22, 142 (2008)
Chen S, Hirota N, Okuda M, Takeguchi M, Kobayashi H, Hanagataa N, Ikom T, Acta Biomater., 7, 644 (2011)

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[2] Brinckmann J, Top. Curr. Chem., 247, 1 (2005)

[3] Aszodi A, Legate KR, Nakchbandi I, Fassler R, Annu. Rev. Cell Dev. Biol., 22, 591 (2006)

[4] Miyagi Y, Chiu LLY, Cimini M, Weisel RD, Radisic M, Weisel RD, Radisic M, Li RK, Biomaterials, 32, 1280 (2011)

[5] Nudelman F, Pieterse K, George A, Bomans PHH, Friedrich H, Brylka LJ, Hilbers PAJ, de With G, Sommerdijk NAJM, Nat. Mater., 9(12), 1004 (2010)

[6] Faraj KA, van Kuppevelt TH, Daamen WF, Tissue Eng., 13, 2387 (2007)

[7] Rudnick A, J. Wound Care, 15, 402 (2006)

[8] Parkman PD, Dev. Biol. Stand., 88, 5 (1996)

[9] Horaud F, Dev. Biol. Stand., 75, 3 (1991)

[10] Celis P, Silvester G, Dev. Biol. Stand., 118, 3 (2004)

[11] Chen S, Hirota N, Okuda M, Takeguchi M, Kobayashi H, Hanagataa N, Ikom T, Acta Biomater., 7, 644 (2011)

[12] Duan R, Zhang J, Du X, Yao X, Konno K, Food Chem., 112, 702 (2009)

[13] Woo JW, Yu SJ, Cho SM, Lee YB, Kim SB, Food Hydrocolloids, 22, 879 (2008)

[14] Arias-Moscoso JL, Soto-Valdez H, Plascencia-Jatomea M, Reyna-Luz Vidal-Quintanar, Rouzaud-Sndez O, Ezquerra-Brauer JM, Polym. Int., 60, 924 (2011)

[15] Nishimoto S, Goto Y, Morishige H, Shiraishi R, Doi M, Akiyama K, Sugahara T, Biosci. Biotechnol. Biochem., 72, 2806 (2008)

[16] Lee K, Park HY, Kim YK, Park JI, Yoon HD, J. Korean Soc. Appl. Biol. Chem., 52, 221 (2009)

[17] Saiga A, Iwai K, Hayakawa T, Takahata Y, Kitamura S, Nishimura T, Morimatsu F, J. Agric. Food Chem., 56, 9586 (2008)

[18] Pati F, Adhikari B, Dhara S, Bioresour. Technol., 101(10), 3737 (2010)

[19] Ko HA, Kim HM, Ha HJ, Kim AR, Jang JE, Kim KH, Lee JH, Song JE, Yoon H, Lee OJ, Lee D, Park CH, Khang G, Inter. J. Tissue Regen., 3, 103 (2012)

[20] Kim HM, Park JY, Kim EY, Song JE, Kwon SY, Chung JW, Khang G, Polym.(Korea), 38(3), 278 (2014)

[21] Kim H, Kim HM, Jang JE, Kim CM, Kim EY, Lee D, Khang G, Int. J. Stem Cells, 6, 67 (2013)

[22] Belbachir K, Noreen R, Gouspillou G, Petibois C, Anal. Bioanal. Chem., 395, 829 (2009)

[23] Han DH, Kang HJ, An JJ, Jo HK, Yoo HR, Seol IC, Kim YS, Korean J. Orient. Physiol. Pathol., 22, 142 (2008)

[24] Chen S, Hirota N, Okuda M, Takeguchi M, Kobayashi H, Hanagataa N, Ikom T, Acta Biomater., 7, 644 (2011)