화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.34, 14-20, February, 2016
DOI10.1016/j.jiec.2015.11.015  Export Citation
Biofunctionalization via flow shear stress resistant adhesive polysaccharide, hyaluronic acid-catechol, for enhanced in vitro endothelialization
Hyunwoo Joo, Eunkyoung Byun1, Mihyun Lee1, Yoonmi Hong, Haeshin Lee1, †, and Pilnam Kim†
  • Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea
  • 1Department of Chemistry, Center for Nature-inspired Technology in KAIST Institute NanoCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea
E-mail:,
We report a single-step method for achieving in vitro endothelialization using a water-resistant adhesive polysaccharide, hyaluronic acid (HA). Introduction of adhesive properties is inspired by the catechol chemistry utilized in marine mussels, in which bioactive hyaluronic acid is chemically modified into hyaluronic acid-catechol (HA-catechol). Quantification of cell adhesiveness to HA-catechol coated substrate was performed with microfluidic device-based adhesion assay under shear forces. The HAcatechol exhibited enhanced ability of surface binding under flow shear force condition compared to unmodified HA. HA-catechol was able to retain the adhered endothelial cells under fluid shear stresses up to 150 dyn/cm2, which is above the physiological condition in human vascular system. This is the first study demonstrating utility of the adhesive force of catechol-tethered polymers under continuous shear force environments such as microfluidic devices. The HA-catechol compound developed herein could provide a straightforward strategy for improving stability in surface retention and preventing substantial tissue damage from the fluctuating blood flow after vascular reconstructive surgery.
Keywords:Biomimetic material;Mussel adhesion;Surface modification;Endothelial cells;Cell adhesion;Hyaluronic acid;Catecholm;Microfluidics
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