BMP-2 immobilization by phosphonated UV-curable low-molecular-weight chitosan derivative on the surface of titanium

Jin-Hong Jeong; Eun-Hye Kim; Ga-Dug Han; Jae-Woon Nah; Yoshihiro Ito; Tae-Il Son

Journal of Industrial and Engineering Chemistry, Vol.34, 33-40, February, 2016

DOI10.1016/j.jiec.2015.10.025 Export Citation

BMP-2 immobilization by phosphonated UV-curable low-molecular-weight chitosan derivative on the surface of titanium

Jin-Hong Jeong, Eun-Hye Kim, Ga-Dug Han, Jae-Woon Nah¹, Yoshihiro Ito², and Tae-Il Son^†

Department of Systems Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Anseong-si, Gyeonggi-do 456-756, Republic of Korea
¹Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Suncheon, Jeollanam-do 540-950, Republic of Korea
²Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan

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The purpose of this study is to immobilize growth factors and coat titanium surfaces using phosphonated UV-curable low-molecular-weight chitosan (Az-P-LM-O-CMC). The structure of the final product was assessed with FT-IR, 31P-NMR, and GPC. The coating of Az-P-LM-O-CMC on titanium was evaluated by measuring water contact angle. Cytotoxicity testing was conducted to determine biocompatibility, and micropatterning was used to check protein immobilization. From the BMP-2 release tests, it was observed that the rate of BMP-2 release could be controlled by multilayer coatings. ALP assay and Ca2+ assay were carried out to evaluate drug immobilization and release effects.

Keywords:Implant coating material;Low molecular weight O-carboxymethyl;Chitosan derivative;Titaniumm,Photo-immobilization

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