Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

A.N. Kapitonov; G.N. Alexandrov; F.D. Vasileva; S.A. Smagulova; V.B. Timofeev; N.R. Maksimova; A.A. Kuznetsov

Korean Journal of Materials Research, Vol.26, No.1, 1-7, January, 2016

DOI10.3740/MRSK.2016.26.1.1 Export Citation

Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

A.N. Kapitonov^†, G.N. Alexandrov, F.D. Vasileva, S.A. Smagulova, V.B. Timofeev, N.R. Maksimova, and A.A. Kuznetsov

M.K. Ammosov North-Eastern Federal University, Yakutsk, 677000 Russia, Russian Federation

E-mail:

The graphene oxides (GOs) were tested as a fluorescent quencher in the field of DNA-diagnostics. The various suspensions of GO nanoplates were prepared by changing the synthesis conditions. The suspensions were stable for at least 6 weeks by differing degrees of functionalization of various oxygen-containing groups of atoms. Depending on the properties of GO nanoplates, their fluorescent quenching abilities, which were determined by the amount of the tagged immobilized oligonucleotide, were also changed. GO suspension synthesized at 75 ℃ of reaction mixture showed the fluorescent quenching of 16.39 nmol/mg, which would be a potential substitution of molecular fluorescent quencher in test-systems for DNAdiagnostics.

Keywords:graphene oxide;DNA diagnostics;fluorescent quencher

[References]

Mustafa B, Muhit R, Neil R, Mehmet VY, ACS Appl. Mater. Inter., 15, 12100 (2014)
Kuznetsov AA, Maksimova NR, Alekxandrov GN, Smagulova SA, Yakut Medical J., 4, 74 (2014)
He S, Song B, Li D, Adv. Funct. Mater., 20, 453 (2012)
Yang HH, Zhu CL, Chen X, Chen L, Angew. Chem.-Int. Edit., 48, 4785 (2009)
Huang J, Liu J, Anal. Chem., 84, 4192 (2012)
Li J, Huang Y, Wang D, Song B, Li Z, Song S, Wang L, Jiang B, Zhao X, Yan J, Liu R, Hec D, Fana C, Chem. Commun., 49, 3125 (2013)
Xiang D, Zheng AH, Luo M, Ji XH, He ZK, Sci. China Chem., 56, 380 (2013)
Li F, Huang Y, Yang Q, Zhong Z, Li D, Wang L, Song S, Fan C, Nanoscale, 2, 1021 (2010)
Xu H, Yang Q, Li F, Tang L, Gao S, Jiang B, Zhao X, Wang L, Fan C, Analyst, 138, 2678 (2013)
Zhao XH, Ma QJ, Wu XX, Zhu X, Anal. Chim. Acta, 727, 67 (2012)
Zhang M, Le HN, Ye BC, ACS Appl. Mater. Inter., 5, 8278 (2013)
Dong H, Gao W, Yan F, Ji H, Ju H, Anal. Chem., 82, 5511 (2010)
Liu M, Zhao H, Chen S, Yu H, Zhang Y, Quan X, Biosens. Bioelectron., 26, 4213 (2011)
Li Z, Zhu W, Zhang J, Jiang J, Shen G, Yu R, Analyst, 138, 3616 (2013)
Hummers WS, Offeman RE, J. Am. Chem. Soc., 80, 1339 (1958)

[Referenced By]

[1] Mustafa B, Muhit R, Neil R, Mehmet VY, ACS Appl. Mater. Inter., 15, 12100 (2014)

[2] Kuznetsov AA, Maksimova NR, Alekxandrov GN, Smagulova SA, Yakut Medical J., 4, 74 (2014)

[3] He S, Song B, Li D, Adv. Funct. Mater., 20, 453 (2012)

[4] Yang HH, Zhu CL, Chen X, Chen L, Angew. Chem.-Int. Edit., 48, 4785 (2009)

[5] Huang J, Liu J, Anal. Chem., 84, 4192 (2012)

[6] Li J, Huang Y, Wang D, Song B, Li Z, Song S, Wang L, Jiang B, Zhao X, Yan J, Liu R, Hec D, Fana C, Chem. Commun., 49, 3125 (2013)

[7] Xiang D, Zheng AH, Luo M, Ji XH, He ZK, Sci. China Chem., 56, 380 (2013)

[8] Li F, Huang Y, Yang Q, Zhong Z, Li D, Wang L, Song S, Fan C, Nanoscale, 2, 1021 (2010)

[9] Xu H, Yang Q, Li F, Tang L, Gao S, Jiang B, Zhao X, Wang L, Fan C, Analyst, 138, 2678 (2013)

[10] Zhao XH, Ma QJ, Wu XX, Zhu X, Anal. Chim. Acta, 727, 67 (2012)

[11] Zhang M, Le HN, Ye BC, ACS Appl. Mater. Inter., 5, 8278 (2013)

[12] Dong H, Gao W, Yan F, Ji H, Ju H, Anal. Chem., 82, 5511 (2010)

[13] Liu M, Zhao H, Chen S, Yu H, Zhang Y, Quan X, Biosens. Bioelectron., 26, 4213 (2011)

[14] Li Z, Zhu W, Zhang J, Jiang J, Shen G, Yu R, Analyst, 138, 3616 (2013)

[15] Hummers WS, Offeman RE, J. Am. Chem. Soc., 80, 1339 (1958)