Two P biofertilizers described by M. Jastrzebska et al. (2016) were enriched with phosphate-solubilizing bacteria (Acidithiobacillus ferrooxidans) and studied for its survivability in soil and the influence of biofertilizers on the total counts of heterotrophic bacteria and fungi, the abundance of earthworms, soil moisture, temp. and pH. The biofertilizers were as effective as superphosphate used for comparison. A. ferrooxidans did not survive in the soil environment of winter wheat. The tested fertilizers did influence neither the total no. of heterotrophic bacteria and fungi in soil, the abundance of earthworms, soil moisture, temp. nor pH.