Nickel oxide nanostructure network electrodes have been fabricated via metal electrodeposition into a silica template on Ni foam as a three-dimensional metal electrode. The metal oxide nanostructure on three-dimensional electrodes provides a pathway to a very high surface area of electrode built on a cheap commercially available material. These electrodes were characterized by electrochemical impedance spectroscopy, field emission scanning electron microscopy, energy-dispersive X-ray analysis and X-ray diffraction pattern. The electrochemical properties of the electrodes, such as the electrochemical active area and some kinetic parameters, were investigated. The prepared electrode was applied as an amperometric sensor for hydrogen peroxide determination in low potential. The presented electrode shows good sensitivity and stability. Finally, the proposed electrochemical sensor was successfully applied to determine H2O2 in milk and the method has been validated using independent estimation by the classical potassium permanganate titration method.