화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.41, No.1, 13-20, January, 2017
DOI10.7317/pk.2017.41.1.13  Export Citation
탈미네랄화된 골분 스펀지에서 쥐지방줄기세포의 골분화 유도
Osteogenic Differentiation of Rat Adipose Stem Cells in Demineralized Bone Particles Sponges
김창현, 장나금, 김도경, 송야성, 송정은, 강길선†
  • 전북대학교 BIN융합공학과, 고분자나노공학과, 고분자융합소재연구소
Chang Hyun Kim, Na Keum Jang, Do Kyung Kim, Yah Seong Song, Jeong Eun Song, and Gilson Khang†
  • Dept. of BIN Convergence Tech & Dept. of PolymerNanoSci Tech, Chonbuk National University, 567 Baekje-daero, Jeonju 54896, Korea
E-mail:
초록
탈미네랄화된 골분(DBP)은 콜라겐, 칼슘 그리고 프로테오글리칸을 포함한다. DBP는 조직공학 분야에서 널리 사용되는 생체재료로, 새로운 뼈나 연골 성장의 기초를 다지는데 도움을 주는 생체 활성 분자를 포함한다. 본 연구에서는 천연 생체재료인 DBP를 1, 2, 3% 비율의 스펀지형태로 제작하였다. 또한 체내 지방에서 쉽게 얻을 수 있는 쥐지방줄기세포(rASCs)를 DBP 스펀지에 파종한 후, 골분화를 확인하기 위해 다음과 같은 연구를 실시하였다. 먼저, 세포의 부착과 증식의 효과를 확인하고자 SEM과 MTT분석을 실시하였으며, 골분화의 발현 정도와 잠재력 확인을 위해 ALP 활성과 RT-PCR을 실시하였다. 조직화학적 염색은 쥐에 DBP 스펀지를 이식한 후 1주, 4주 및 8주 후에 적출하여, Alizarine Red S 염색과 Von Kossa 염색을 통해, 생체 내에서 골분화의 효과를 확인하였다. 그 결과, 1% DBP 스펀지가 rASCs의 골분화능을 증가시키는데 적합한 환경을 제공한다는 것을 확인할 수 있었다.
Demineralized bone particle (DBP) is a well-studied biomaterial, composed of collagen, calcium and proteoglycan. It is widely used in tissue engineering and regenerative medicine, because it contains powerful bioactive molecules that facilitate new bone or cartilage growth. Herein, we fabricated 1, 2 and 3% sponges using natural biomaterial DBP and studied its efficiency in rat adipose stem cells (rASCs) as models. After rASCs were seeded on DBP sponges, we evaluated rASCs adhesion, proliferation, cell attachment, proliferation and osteogenesis using SEM, MTT, and bone differentiation expose using ALP assay, RT-PCR in vitro. Histological examinations were performed at 1, 4 and 8 weeks after implantation DBP sponge to assess the effect of bone differentiation in vivo using Alizarin Red S, Von Kossa staining. We found that 1% DBP sponge can provide suitable environment for bone differentiation of rASCs.
Keywords:rat adipose stem cell;demineralized bone particle;sponge;osteogenesis;bone differentiation
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