화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.10, No.3, 158-167, June, 2002
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Preparation and Characterization of Small Intestine Submucosa Powder Impregnated Poly(L-lactide) Scaffolds: The Application for Tissue Engineered Bone and Cartilage
Gilson Khang†, John M. Rhee, Philkyung Shin1, In Young Kim1, Bong Lee1, Sang Jin Lee2, Young Moo Lee2, Hai Bang Lee3, and Ilwoo Lee4
  • Department of Polymer Science and Technology, Chonbuk National University, 664-14, Dukjin Dong 1 Ga, Dukjin Ku, Chonju 561-756, Korea
  • 1Department of Polymer Engineering, Bukyung National University, San 100, Yongdang Dong, Nam Ku, Busan 608-023, Korea
  • 2Department of Industrial Chemistry, Hanyang University, 17 Haengdang Dong, Seongdong Ku, Seoul 133-791, Korea
  • 3Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yusung, Daejon 305-606, Korea
  • 4Department of Neurosurgery, Catholic University Medical College, 520-2 Deaheung Dong, Jung Ku, Daejon 301-723, Korea
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In order to endow with new bioactive functionality from small intestine submucosa (SIS) powder as natural source to poly(L-lactide) (PLA) and poly(lactide-co-glycolide) (PLGA) synthetic biodegradable polymer, porous SIS/PLA and SIS/PLGA as natural/synthetic composite scaffolds were prepared by means of the solvent casting/salt leaching methods for the possibility of the application of tissue engineered bone and cartilage. A uniform distribution of good interconnected pores from the surface to core region was observed the pore size of 40~500 μm independent with SIS amount using the solvent casting/salt leaching method. Porosities, specific pore areas as well as pore size distribution also were almost same. After the fabrication of SIS/PLA hybrid scaffolds, the wetting properties was greatly enhanced resulting in more uniform cell seeding and distribution. Five groups as PGA nonwoven mesh without glutaraldehyde (GA) treatment, PLA scaffold without or with GA treatment, and SIS/PLA (Code No. 3 ;1: 12 of salt content, 0.4 : 1 of SIS content, and 144 μm of median pore size) without or with GA treatment were implanted into the back of nude mouse to observe the effect of SIS on the induction of cells proliferation by hematoxylin and eosin, and von Kossa staining for 8 weeks. It was observed that the effect of SIS/PLA scaffolds with GA treatment on bone induction are stronger than PLA scaffolds, that is to say, in the order of PLA/SIS scaffolds with GA treatment > PLA/SIS scaffolds without GA treatment > PGA nonwoven > PLA scaffolds only with GA treatment = PLA scaffolds only without GA treatment for the osteoinduction activity. The possible explanations are (1) many kinds of secreted, circulating, and extracellular matrix-bound growth factors from SIS to significantly affect critical processes of tissue development and differentiation, (2) the exposure of SIS to GA resulted in significantly calcification, and (3) peri-implant fibrosis due to covalent bonding between collagen molecule by crosslinking reaction. In conclusion, it seems that SIS plays an important role for bone induction in SIS/PLA scaffolds for the application of tissue engineering area.
Keywords:SIS;PLA;tissue engineering;bone;scaffolds
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