감마선을 이용한 돼지 무세포 연골세포외기질이 함유된 CMC 수화젤의 제조 및 특성 연구

정성린; 박종석; 권희정; 안성준; 임윤묵; 송보람; 김영직; 민병현; 김문석; Sung In Jeong; Jong-Seok Park; Hui-Jeong Gwon; Sung-Jun An; Youn-Mook Lim; Bo Ram Song; Young Jick Kim; Byoung-Hyun Min; Moon Suk Kim

Polymer(Korea), Vol.41, No.1, 151-156, January, 2017

DOI10.7317/pk.2017.41.1.151 Export Citation

감마선을 이용한 돼지 무세포 연골세포외기질이 함유된 CMC 수화젤의 제조 및 특성 연구

Preparation and Characterization of CMC Hydrogels with Porcine Cartilage Acellular Matrix Using Gamma-Irradiation

정성린^{1, †}, 박종석¹, 권희정¹, 안성준¹, 임윤묵^{1, †}, 송보람², 김영직², 민병현^{2, 3}, 김문석⁴

¹한국원자력연구원 첨단방사선연구소 공업환경연구부
²아주대학교 세포치료센터
³아주대학교 의과대학 정형외과교실
⁴아주대학교 분자과학기술과

Sung In Jeong^{1, †}, Jong-Seok Park¹, Hui-Jeong Gwon¹, Sung-Jun An¹, Youn-Mook Lim^{1, †}, Bo Ram Song², Young Jick Kim², Byoung-Hyun Min^{2, 3}, and Moon Suk Kim⁴

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 56212, Korea
²Cell Therapy Center, Ajou University Medical Center, Suwon 16499, Korea
³Department of Orthopedic Surgery, Ajou University School of Medicine, Suwon 16499, Korea
⁴Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea

E-mail:,

초록

본 연구는 감마선을 이용하여 가교된 유착방지용 carboxymethyl cellulose sodium salt(CMC-Na)와 porcine cartilage acellular matrix(PCAM) 수화젤을 제조하였다. CMC는 높은 농도의 수용액에서 감마선에 의해 가교가 되는 천연고분자 중에 하나이다. 방사선 가교된 CMC/PCAM 수화젤의 물리/화학적 특성을 알기 위해 SEM, 젤화율, 젤강도, 팽윤도, FTIR-ATR 등 분석을 통해 특성을 확인하였다. 감마선으로 가교된 CMC/PCAM 수화젤에서 PCAM 비율이 증가할수록 젤화율과 젤강도는 감소하였고 팽윤도는 증가하였다. 또한 CMC/PCAM 수화젤에서 세포의 부착도와 증식도가 CMC 수화젤에 비해 낮음을 확인하였다. 결론적으로, 본 연구에서는 방사선으로 가교된 CMC/PCAM 수화젤은 유착방지를 위한 유착방지제로서 응용이 기대된다.

In this study, a novel crosslinked carboxymethyl cellulose sodium salt (CMC) and porcine cartilage acellular matrix (PCAM) hydrogel for adhesion prevention were prepared by gamma-irradiation. CMC is known as a kind of cross-linked natural polymer at highly concentrated aqueous solution under action of gamma-irradiation. The radiationcrosslinked CMC/PCAM hydrogels were characterized by scanning electron microscope (SEM), gel-fraction, gel strength, swelling behavior, and FTIR-ATR spectroscopy. The results showed that the PCAM addition increased the swelling behavior and decreased the gel-fraction and gel strength of CMC/PCAM hydrogels. The degree of attachment and proliferation of human fibroblast on CMC/PCAM hydrogel was lower than the CMC hydrogels. In conclusion, this study suggests that the radiation cross-linked CMC/PCAM hydrogels may be useful as an anti-adhesion barrier.

Keywords:carboxymethyl cellulose sodium salt;porcine cartilage acellular matrix;hydrogel;gamma-irradiation;crosslinking;adhesion prevention

[References]

Dizeregal GS, Campeau JD, Hum. Reprod. Update, 7, 547 (2001)
Ito T, Yeo Y, Highley CB, Bellas E, Kohane DS, Biomaterials, 28, 3418 (2007)
Boland GM, Weigel RJ, J. Surg. Res., 132, 3 (2006)
Risberg B, Eur. J. Surg. Suppl., 577, 32 (1997)
Sugarbaker PH, Cancer Treat. Res., 81, 75 (1996)
Monk B, Fowler JM, Burger RA, McGonigle KF, Eddy GL, Montz FJ, Int. J. Gynecol. Cancer, 8, 403 (1998)
Burns JW, Colt MJ, Burgees LS, Skinner KC, Eur. J. Surg. Suppl., 577, 40 (1997)
Kim J, Shin IS, Lee KY, Polym. Korea, 40(3), 397 (2016)
Rosiak JM, J. Control. Release, 31, 9 (1994)
Weis C, Odermatt EK, Kressler J, Funke Z, Wehner T, Freytag D, J. Biomed. Mater. Res., 70, 191 (2004)
Lauder CI, Garcea G, Strickland A, Madden GJ, J. Surg. Res., 171, 877 (2011)
Grainger DA, Meyer WR, DeCherney AH, Diamond MP, J. Gynecol. Surg., 7, 97 (1991)
Zhang Z, Ni J, Chen L, Yu L, Xu J, Ding J, Biomaterials, 32, 4725 (2011)
Liu Y, Li H, Shu XZ, Gray SD, Prestwich GD, Fertil. Steril., 83, 1275 (2005)
Ricaurte E, Hilgers TW, J. Reprod. Med., 34, 535 (1989)
Liu LS, Berg RA, J. Biomed. Mater. Res., 63, 326 (2002)
Miyamato J, Takahashi S, Ito H, Inagaki H, J. Biomed. Mater. Res., 23, 125 (1989)
Peck LS, Quigg JM, Fossum GT, Goldberg EP, J. Invest. Surg., 8, 337 (1995)
Burns JW, Colt MJ, Burgees LS, Skinner KC, Eur. J. Surg. Suppl., 577, 40 (1997)
Lee JH, Nho YC, Lim YM, Son TI, J. Appl. Polym. Sci., 96(4), 1138 (2005)
Adel M, Abou-Youssef H, El-Gendy AA, Nada AM, J. Nat. Sci., 8, 8 (2010)
Feng X, Pelton R, J. Macromol. Sci.-Polym. Rev, 40, 1624 (2007)
Sung HW, Huang DM, Chang WH, Huang RN, Hsu JC, J. Biomed. Mater. Res., 46, 520 (1999)
Vercruysse KP, Marecak DM, Marecek JF, Prestwich GD, Bioconjugate Chem., 8, 686 (1997)
Kim AR, Park HS, Kim SS, Noh I, Biomater. Res., 17, 181 (2013)
Leavit FC, J. Polym. Sci., 45, 536 (1960)
Leavit FC, J. Polym. Sci., 51, 349 (1960)
Rosiak JM, Ulanski P, Radiat. Phys. Chem., 55, 139 (1999)
Liu P, Peng J, Li J, Wu J, Radiat. Phys. Chem., 72, 635 (1999)
Choi BH, Choi KH, Lee HS, Song BR, Park SR, Yang JW, Min BH, Biomaterials, 35, 5711 (2014)
Baek JH, Kim K, Yang SS, Park SH, Song BR, Yun HW, Jeong SI, Kim YJ, Min BH, Kim MS, Materials, 9, 49 (2016)
Fei I, Wach RA, Mitomo H, Yoshii F, Kume T, J. Appl. Polym. Sci., 78(2), 278 (2000)
Jo SY, Lim YM, Youn MH, Gwon HJ, Park JS, Nho YC, Shin H, Polym. Korea, 33(6), 551 (2009)
Boskey A, Camacho NP, Biomaterials, 28, 2465 (2007)

[1] Dizeregal GS, Campeau JD, Hum. Reprod. Update, 7, 547 (2001)

[2] Ito T, Yeo Y, Highley CB, Bellas E, Kohane DS, Biomaterials, 28, 3418 (2007)

[3] Boland GM, Weigel RJ, J. Surg. Res., 132, 3 (2006)

[4] Risberg B, Eur. J. Surg. Suppl., 577, 32 (1997)

[5] Sugarbaker PH, Cancer Treat. Res., 81, 75 (1996)

[6] Monk B, Fowler JM, Burger RA, McGonigle KF, Eddy GL, Montz FJ, Int. J. Gynecol. Cancer, 8, 403 (1998)

[7] Burns JW, Colt MJ, Burgees LS, Skinner KC, Eur. J. Surg. Suppl., 577, 40 (1997)

[8] Kim J, Shin IS, Lee KY, Polym. Korea, 40(3), 397 (2016)

[9] Rosiak JM, J. Control. Release, 31, 9 (1994)

[10] Weis C, Odermatt EK, Kressler J, Funke Z, Wehner T, Freytag D, J. Biomed. Mater. Res., 70, 191 (2004)

[11] Lauder CI, Garcea G, Strickland A, Madden GJ, J. Surg. Res., 171, 877 (2011)

[12] Grainger DA, Meyer WR, DeCherney AH, Diamond MP, J. Gynecol. Surg., 7, 97 (1991)

[13] Zhang Z, Ni J, Chen L, Yu L, Xu J, Ding J, Biomaterials, 32, 4725 (2011)

[14] Liu Y, Li H, Shu XZ, Gray SD, Prestwich GD, Fertil. Steril., 83, 1275 (2005)

[15] Ricaurte E, Hilgers TW, J. Reprod. Med., 34, 535 (1989)

[16] Liu LS, Berg RA, J. Biomed. Mater. Res., 63, 326 (2002)

[17] Miyamato J, Takahashi S, Ito H, Inagaki H, J. Biomed. Mater. Res., 23, 125 (1989)

[18] Peck LS, Quigg JM, Fossum GT, Goldberg EP, J. Invest. Surg., 8, 337 (1995)

[19] Burns JW, Colt MJ, Burgees LS, Skinner KC, Eur. J. Surg. Suppl., 577, 40 (1997)

[20] Lee JH, Nho YC, Lim YM, Son TI, J. Appl. Polym. Sci., 96(4), 1138 (2005)

[21] Adel M, Abou-Youssef H, El-Gendy AA, Nada AM, J. Nat. Sci., 8, 8 (2010)

[22] Feng X, Pelton R, J. Macromol. Sci.-Polym. Rev, 40, 1624 (2007)

[23] Sung HW, Huang DM, Chang WH, Huang RN, Hsu JC, J. Biomed. Mater. Res., 46, 520 (1999)

[24] Vercruysse KP, Marecak DM, Marecek JF, Prestwich GD, Bioconjugate Chem., 8, 686 (1997)

[25] Kim AR, Park HS, Kim SS, Noh I, Biomater. Res., 17, 181 (2013)

[26] Leavit FC, J. Polym. Sci., 45, 536 (1960)

[27] Leavit FC, J. Polym. Sci., 51, 349 (1960)

[28] Rosiak JM, Ulanski P, Radiat. Phys. Chem., 55, 139 (1999)

[29] Liu P, Peng J, Li J, Wu J, Radiat. Phys. Chem., 72, 635 (1999)

[30] Choi BH, Choi KH, Lee HS, Song BR, Park SR, Yang JW, Min BH, Biomaterials, 35, 5711 (2014)

[31] Baek JH, Kim K, Yang SS, Park SH, Song BR, Yun HW, Jeong SI, Kim YJ, Min BH, Kim MS, Materials, 9, 49 (2016)

[32] Fei I, Wach RA, Mitomo H, Yoshii F, Kume T, J. Appl. Polym. Sci., 78(2), 278 (2000)

[33] Jo SY, Lim YM, Youn MH, Gwon HJ, Park JS, Nho YC, Shin H, Polym. Korea, 33(6), 551 (2009)

[34] Boskey A, Camacho NP, Biomaterials, 28, 2465 (2007)