화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.26, No.1, 1-8, January, 2018
DOI10.1007/s13233-018-6002-7  Export Citation
Microwell-mediated cell spheroid formation and its applications
Yu-Shik Hwang, Jinseok Kim1, Hee Jeong Yoon2, Ji In Kang3, Ki-Ho Park3, and Hojae Bae4, †
  • Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea
  • 1The Center for Bionics, Korea Institute of Science and Technology, Seoul 02792, Korea
  • 2College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea
  • 3Department of Dentistry, Graduate School, Kyung Hee University, Seoul 02447, Korea
  • 4KU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea
E-mail:
There is a continuing attempt to study cell to cell interactions to control the growth, function, and differentiation of cells. One approach that can be utilized to assess representative cell to cell interactions is by using three-dimensional cell spheroids, also referred to as cell aggregates, generated by adhering cells to each other through cell-adhesion molecules such as cadherin. Compared with conventional two-dimensional cell monolayer, cell spheroid offers more realistic platform to predict cell behavior in high-throughput manner. To recapitulate the cell spheroid formation in vitro, microwell-mediated culture system has become a robust and efficient tool for providing uniform-sized spheroids. In this review, we first iterate the recent developments and innovations in microwell-mediated cell culture platform, focusing on formation and function of cell spheroids using various cells such as embryonic stem cells, postnatal stem cells, and somatic cells. Furthermore, the recent advancements in applications of cell spheroids generated from microwell-mediated culture system is covered in this article. The discussion on the integrative biology regarding cell to cell interaction and other biological events in cell spheroid is another focal point of this review.
Keywords:microwell-mediated differentiation;cell spheroid;differentiation;embryonic stem cell;postnatal stem cell;somatic cell
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