beta-Cyclodextrin (CD) gels crosslinked by epichlorohydrin (CD-gel) were modified with poly(N-isopropylacrylamide) (PIPA; M-n 4900) chains. The CD residue in the gel associated with 8-anilino-1-naphthalenesulfonic acid (ANS) more strongly than the free PIPA-carrying CD. The van’t Hoff plot for complexation of ANS with the gel drifted largely from linear relationship above the coil-globule transition temperature of individual PIPA chains. Furthermore, a relatively more significant temperature effect on the stereoselective adsorption of phenylalanine to the CD gel was observed by the modification with PIPA chains. These results imply that the ability of the CD-gel-PIPA conjugate to form inclusion complex could be skillfully controlled by temperature.