LPS로 자극된 RAW264.7 대식세포에서 우슬 및 땅두릅 복합 발효추출물의 항염증 효과

우영민; 조은솔; 김옥주; 이영호; 안미영; 이동근; 이상현; 하종명; 김안드레; Young Min Woo; Eun Sol Jo; Ok Ju Kim; Young-Ho Lee; Mee Young Ahn; Dong-Geun Lee; Sang-Hyeon Lee; Jong-Myung Ha; Andre Kim

Applied Chemistry for Engineering, Vol.30, No.4, 479-486, August, 2019

DOI10.14478/ace.2019.1049 Export Citation

LPS로 자극된 RAW264.7 대식세포에서 우슬 및 땅두릅 복합 발효추출물의 항염증 효과

Anti-inflammatory Effects of Achyranthes japonica Nakai and Aralia continentalis Kitagawa Complex Fermented Extracts on LPS-stimulated RAW264.7 Macrophage

우영민¹, 조은솔¹, 김옥주¹, 이영호^{2, 3}, 안미영⁴, 이동근⁴, 이상현⁴, 하종명⁴, 김안드레^{4, †}

¹신라대학교 자연과학연구소
²한국기초과학연구소
³과학기술연합대학원
⁴신라대학교 제약공학과

Young Min Woo¹, Eun Sol Jo¹, Ok Ju Kim¹, Young-Ho Lee^{2, 3}, Mee Young Ahn⁴, Dong-Geun Lee⁴, Sang-Hyeon Lee⁴, Jong-Myung Ha⁴, and Andre Kim^{4, †}

¹Natural Science Institute, Silla University, Busan 46958, South Korea
²Protein Structure Research Group, Division of Bioconvergence Analysis, Korea Basic Science Institute, Chungcheongbuk-do 28119, South Korea
³Bio-Analytical Science, University of Science and Technology (UST), Daejeon 34113, South Korea
⁴Department of Pharmaceutical Engineering, Silla University, Busan 46958, South Korea

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초록

본 연구는 RAW264.7 대식세포에 대한 우슬과 땅두릅 복합 열수추출물(1 : 2, 1 : 3, 1 : 5, 2 : 1, 3 : 1 및 5 : 1 비율)의 항염증 효과를 조사했다. 세포 독성은 CCK 분석을 사용하여 확인하였다. 우리는 ELISA 키트를 사용하여 IL-1β, IL-6 및 TNFα를 측정함으로써 우슬 및 땅두릅 복합 열수추출물의 항염증 효과를 평가하였다. 우슬 및 땅두릅 복합 열수추출물은 LPS로 자극된 RAW264.7 대식세포에서 IL-1β 및 TNFα를 유의적으로 억제하였다. 복합 추출물의 다양한 비율을 비교하였을 때, 2 : 1 비율은 LPS로 유도된 RAW264.7 세포에서 훨씬 더 효능이 있었고 TNFα의 생성 또한 억제하였다. 본 연구의 결과는 우슬 및 땅두릅 복합 열수추출물이 RAW264.7 대식세포에 대해 강력한 항염증 효과를 갖는 다는 것을 보여주며, 이들 추출물은 염증 질환 예방을 위한 기능성 식품의 좋은 공급원이 될 수 있다고 사료된다.

This study investigated the anti-inflammatory effects of mixed extracts of Achyranthes japonica Nakai (Aj) and Aralia continentalis Kitagawa (Ac) (ratios of 1 : 2, 1 : 3, 1 : 5, 2 : 1, 3 : 1 and 5 : 1) on RAW264.7 macrophages. Cell toxicity was determined using a cell counting kit (CCK) assay. We evaluated anti-inflammatory effects of the mixed extracts of Aj and Ac by measuring interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)α using an enzyme-linked immunosorbent assay (ELISA) kit assay. The mixed extracts of Aj and Ac inhibited lipopolysaccharide (LPS)-induced IL-1β and TNFα in LPS-stimulated macrophages. Comparing different ratios of the mixed extracts, the 2 : 1 ratio of Aj and Ac has much more potency and inhibited the production of TNFα in LPS-induced RAW264.7 cells. The results of the present study showed that the mixed extracts of Aj and Ac have potential anti-inflammatory effects on RAW264.7 macrophages. Therefore, these extracts may be used as a good source of functional foods for the protection against inflammatory diseases.

Keywords:Achyranthes japonica Nakai (Aj);Aralia continentalis Kitagawa (Ac);Anti-inflammatory;Macrophage

[References]

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Choi YK, Sul JU, Park SK, Yu SN, Kim SH, Rhee MS, Shin MS, J. Life Sci., 22, 1729 (2012)
Lee SG, Lee EJ, Park WD, Kim JB, Choi SW, Korean J. Food Sci. Technol., 45, 624 (2011)
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Park JH, Kang SN, Shin D, Hur IC, Kim IS, Jin SK, J. Anim. Sci., 26, 287 (2013)
Han GJ, Shin DS, Jang MS, Korean J. Food Sci. Technol., 40, 680 (2008)
Bosca L, Zeini M, Traves PG, Hortelano S, Toxicology, 208, 249 (2005)
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Cho UM, Hwang HS, J. Soc. Cosmet. Sci. Korea, 43, 157 (2017)
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[2] Kim CH, Lee MA, Kim TW, Jang JY, Kim HJ, J. Korean Soc. Food Sci. Nutr., 41, 1645 (2012)

[3] Moro C, Palacios I, Lozano M, Arrigo M, Guillamon E, Villares A, Lafuente AG, Food Chem., 130, 350 (2012)

[4] Baker RG, Hayden MS, Ghosh S, Cell Metab., 13, 11 (2011)

[5] Zhang G, Ghosh S, J. Endotoxin Res., 6, 453 (2000)

[6] Park HJ, Song MJ, Prev. Nutr. Food Sci., 22, 50 (2017)

[7] Choi YK, Sul JU, Park SK, Yu SN, Kim SH, Rhee MS, Shin MS, J. Life Sci., 22, 1729 (2012)

[8] Lee SG, Lee EJ, Park WD, Kim JB, Choi SW, Korean J. Food Sci. Technol., 45, 624 (2011)

[9] Cho HS, Kang SW, Kim JH, Choi MJ, Yu HW, Park E, Chun HS, Korean Soc. Biotechnol. Bioeng J., 29, 29 (2004)

[10] Bang SY, Kim JH, Kim HY, Lee YJ, Park SY, Lee SJ, Kim YH, J. Ethnopharmacol., 144, 109 (2012)

[11] Jeong HY, Moh SH, Yang Y, Yu T, Oh J, Jeong D, Cho JY, J. Ethnopharmacol., 143, 746 (2012)

[12] Lim H, Jung HA, Choi JS, Kim YS, Kang SS, Kim HP, Arch. Pharm. Res., 32, 1237 (2009)

[13] Jo ES, Woo YM, Kim OJ, Jo MY, Ahn MY, Lee JH, Ha JM, Kim A, Appl. Chem. Eng., 30(2), 145 (2019)

[14] Woo YM, Kim OJ, Jo ES, Jo MY, Ahn MY, Lee SH, Ha JM, Kim A, J. Life Sci., 28, 1438 (2018)

[15] Kang DH, Kim HS, Korean J. Microbiol. Biotechnol., 39, 259 (2011)

[16] Chung TH, Shim KS, Kim DS, Lee JH, Ma JY, J. Korean Orient. Med., 32, 67 (2011)

[17] Choi YJ, Kim SW, Jang JK, Park YS, Park H, Shim KS, Lee HS, Chung MS, Food Eng. Prog., 13, 1 (2009)

[18] Choi WS, Kwon HS, Lim HW, No RW, Lee HY, Korean J. Microbiol. Biotechnol., 41, 183 (2013)

[19] Lin JY, Tang CY, Food Chem., 101, 140 (2007)

[20] Park JH, Kang SN, Shin D, Hur IC, Kim IS, Jin SK, J. Anim. Sci., 26, 287 (2013)

[21] Han GJ, Shin DS, Jang MS, Korean J. Food Sci. Technol., 40, 680 (2008)

[22] Bosca L, Zeini M, Traves PG, Hortelano S, Toxicology, 208, 249 (2005)

[23] Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR, Anal. Biochem., 126, 131 (1982)

[24] Aeberhard EE, Henderson SA, Arabolos NS, Griscavage JM, Castro FE, Barrett CT, Ignarro LJ, Biochem. Biophys. Res. Commun., 208, 1053 (1995)

[25] Cho UM, Hwang HS, J. Soc. Cosmet. Sci. Korea, 43, 157 (2017)

[26] Lee HJ, Shin JS, Lee WS, Shim HY, Park JM, Jang DS, Lee KT, Biol. Pharm. Bull., 39, 657 (2016)

[27] Kim KR, Jeong CK, Park KK, Choi JH, Park JH, Lim SS, Chung WY, J. Biomed. Biotechnol., 2010, 709378 (2010)

[28] Kang CH, Koo JR, So JS, Korean J. Food Sci. Technol., 44, 621 (2012)

[29] Lee SG, Jo DJ, Chang HJ, Kang H, J. Naturopathy, 4, 10 (2015)

[30] Kang MJ, Hwang CR, Lee SJ, J. Agric. Life Sci., 49, 279 (2015)