화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.30, No.6, 681-686, December, 2019
DOI10.14478/ace.2019.1078  Export Citation
Clostridium autoethanogenum을 이용한 합성가스 발효에 대한 비타민과 황공급원의 영향
Effect of Vitamin and Sulfur Sources on Syngas Fermentation Using Clostridium autoethanogenum
임홍래, 안태광, 박소은1, 김영기†
  • 한경대학교 화학공학과
  • 1한경대학교 화학기술연구소
Hongrae Im, Taegwang An, Soeun Park1, and Young-Kee Kim†
  • Department of Chemical Engineering, Hankyong National University, Anseong, Gyeonggi-do 17579, Korea
  • 1Research Center of Chemical Technology, Hankyong National University, Anseong, Gyeonggi-do 17579, Korea
E-mail:
초록
이 연구에서는 합성가스를 기질로 사용하는 Clostridium autoethanogenum 배양 공정에서 에탄올 생산성 향상을 위하여 배지 성분의 농도가 균주 성장과 에탄올 생산에 미치는 영향을 조사하였다. C. autoethanogenum 배양에 사용되는 기본배지 구성 성분 중 비타민 종류인 D-Ca-pantothenate, vitamin B12와 황 공급원인 sodium sulfide를 조사 대상 성분으로 선정하여 이 성분들의 농도를 달리하였을 때 균주 성장과 에탄올 생산에 미치는 영향을 확인하였다. D-Ca-pantothenate 는 0.5, 5, 50, 500 mg/L 농도를 시험하였으며 0.5 mg/L에서 에탄올 생산량이 약간 증가하는 경향을 보였지만 시험한 농도 범위에서 균주의 성장이나 에탄올 생산에 대한 주목할 만한 영향은 관찰되지 않았다. Vitamin B12는 0.1, 1.0, 10, 100 mg/L의 농도범위에서 균주 성장과 에탄올 생산에 미치는 영향을 관찰하였으며, 0.1 mg/L 농도에서 에탄올 생산농도가 일반적 기본배지 농도인 10 mg/L에서보다 245% 증가되는 것을 확인하였다. Sodium sulfide는 0.5, 5, 10 g/L 농도범위에서 균주 성장과 에탄올 생산에 미치는 영향을 분석하였으며, 기본배지의 사용농도인 0.5 g/L을 초과하여 과잉 공급하였을 때, 균주 성장 저해 현상이 관찰되었다. 결과적으로 연구에 사용된 세 가지 배지 성분 중, D-Ca-pantothenate 와 sodium sulfide는 배지 내 성분 농도에 따른 에탄올 생산농도 향상을 이루지 못하였으나, vitamin B12는 기본 배지 내일반적인 농도의 1/100을 사용함으로써 에탄올 생산농도 향상을 이룰 수 있었다.
In this work, the effect of the culture medium composition on microbial growth and ethanol production in Clostridium autoethanogenum culture was investigated to enhance the ethanol productivity. D-Ca-pantothenate, vitamin B12 (as vitamins), and sodium sulfide (as sulfur source) were selected as examined components, and the effects of components’ concentration on cell growth and ethanol production was investigated. For D-Ca-pantothenate concentrations varing from 0.5, 5, 50 and 500 mg/L, a slight increase in the ethanol production was observed at the 0.5 mg/L, but negligible differences in microbial growth and ethanol production were measured for the concentration ranges examined. The effect of vitamin B12 concentrations from 0.1, 1.0, 10, and 100 mg/L on the microbial growth and ethanol production was investigated, and it was found that the ethanol production using a 0.1 mg/L of vitamin B12 concentration increased by 245% compared to that of using the basic medium concentration (10 mg/L). The effect of sodium sulfide concentrations (0.5, 5, and 10 g/L) on the microbial growth and ethanol production was also studied, and the inhibition of microbial growth was observed when the sodium sulfide usage was over 0.5 g/L. In conclusion, changes in D-Ca-pantothenate and sodium sulfide concentrations did not affect the ethanol production, whereas even a 100 times lower concentration of vitamin B12 than that of the basic medium improved the production.
Keywords:Clostridium autoethanogenum;Syngas fermentation;Pantothenate;Vitamin B12;Sodium sulfide
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