화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.82, 234-242, February, 2020
DOI10.1016/j.jiec.2019.10.018  Export Citation
Influence of PEG chain length on colloidal stability of mPEGylated polycation based coacersomes for therapeutic protein delivery
Heejung Jo, Mani Gajendiran1, and Kyobum Kim1, †
  • Department of Bioengineering, College of Lifesciences and Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea
  • 1Department of Chemical and Biochemical Engineering, Dongguk University, Seoul, 04620, Republic of Korea
E-mail:
The polycation/polyanion based coacervate platform for therapeutic protein delivery finds difficulty in their colloidal stability under physiological environment. To overcome this issue, PEGylation could be a versatile strategy to enhance colloidal stability. Herein, a poly(ethylene arginyl aspartate diglyceride) (PEAD) polycation was synthesized, and a series of methoxy polyethylene glycols (mPEG) including mPEG350, mPEG750 and mPEG2000 were attached with PEAD polycation to obtain mPEG350-PEAD, mPEG750-PEAD and mPEG2000-PEAD respectively. The PEAD and mPEGylated PEADs were complexed with heparin (HEP) to fabricate coacervate (Coa) and coacersome (mP_Coa) particles respectively. The colloidal stability of Coa and mP_Coa coacersomes has been investigated by dynamic light scattering (DLS) and microscopic techniques. The vascular endothelial growth factor 165 (VEGF-165) was encapsulated in the Coa or mP_Coa particles and administered to human umbilical vein endothelial cells (HUVECs) to induce a tubular network formation in vitro. All the polycations are highly biocompatible and exhibit more than 94 % of VEGF-165 loading efficiency. An effect of mPEG chain length on colloidal stability of mP_Coa and in vitro tubular formation ability of HUVECs has been investigated.
Keywords:mPEGylation;Coacersome;Colloidal stability;Protein delivery;Polycation
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