Non-ionic polysorbate-based nanoparticles for efficient combination chemo/photothermal/photodynamic therapy

Vasanthan Ravichandran; Thuy Giang Nguyen Cao; Dae Gun Choi; Han Chang Kang; Min Suk Shim

Journal of Industrial and Engineering Chemistry, Vol.88, 260-267, August, 2020

DOI10.1016/j.jiec.2020.04.023 Export Citation

Non-ionic polysorbate-based nanoparticles for efficient combination chemo/photothermal/photodynamic therapy

Vasanthan Ravichandran, Thuy Giang Nguyen Cao, Dae Gun Choi, Han Chang Kang¹, and Min Suk Shim^†

Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea
¹Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomicsbased Future Pharmacy, College of Pharmacy, The Catholic University of Korea, Gyeonggi-do 14662, Republic of Korea

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Combination therapy has gained great attraction as an effective strategy for the treatment of cancers. In this study, non-ionic polysorbate-based nanoparticles were prepared for combination chemo/photo-thermal/photodynamic cancer therapy via effective intracellular delivery of piperlongumine (PL) and indocyanine green (ICG). PL was used as a pro-oxidant drug that can induce selective apoptosis through the increase of oxidative stress in cancer cells. ICG was used as a near-infrared (NIR) light-absorbing photothermal and photodynamic agent. Non-ionic Tween 80 (T80) polysorbate was used to prepare PLand ICG-loaded micelles with nanoscale diameters. Both PL and ICG were efficiently encapsulated in the T80-based micelles. Storage stability of ICG in aqueous solutions was greatly improved by encapsulation into the T80-based micelles. ICG-loaded T80 micelles (ICG-T80) showed higher photothermal conversion efficiency than free ICG. T80 also increased cellular uptake of ICG. Moreover, ICG-T80 showed NIR lighttriggered reactive oxygen species (ROS) generation in MCF-7 human breast cancer cells, owing to the photodynamic effects of NIR light-absorbing ICG. PL-loaded T80 micelles also increased intracellular ROS levels in MCF-7 cells owing to the pro-oxidant activity of PL. In vitro study showed that ICG-T80 micelles exhibited efficient anticancer activity under NIR irradiation via combined photothermal therapy (PTT) and photodynamic therapy (PDT). PL- and ICG-loaded T80 micelles (PL-ICG-T80) further increased the cytotoxicity via combined chemo/PTT/PDT. PL-ICG-T80 also showed cancer-selective cytotoxicity. This study demonstrates that PL-ICG-T80 micelles are effective for NIR light-triggered combination chemo/ PTT/PDT.

Keywords:Polysorbate;Tween 80;Cancer therapy;Combination therapy;Piperlongumine;Indocyanine green

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[2] Cao J, Chen Z, Chi J, Sun Y, Sun Y, Artif. CellsNanomed. Biotechnol., 46, 817 (2018)

[3] Luo D, Carter KA, Miranda D, Lovell JF, Chemophototherapy: Adv. Sci., 4, 160010 (2017)

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[8] Kuo WS, Chang YT, Cho KC, Chiu KC, Lien CH, Yeh CS, Chen SJ, Biomaterials, 33, 3270 (2012)

[9] Barth BM, Altinoglu EI, Shanmugavelandy SS, Kaiser JM, et al., ACS Nano, 5, 5325 (2011)

[10] Zheng X, Xing D, Zhou F, Wu B, Chen WR, Mol. Pharm., 8, 447 (2011)

[11] Li A, Wang Y, Chen T, Zhao W, Zhang A, Feng S, Liu J, Eur. Polym. J., 92, 51 (2017)

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[14] Sheng Z, Hu D, Zheng M, Zhao P, Liu H, Gao D, Gong P, Gao G, Zhang P, Ma Y, Cai L, ACS Nano, 8, 12310 (2014)

[15] Jiao J, Adv. Drug Deliv. Rev., 60, 1663 (2008)

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[17] Regev R, Assaraf YG, Eytan GD, Eur. J. Biochem., 259, 18 (1999)

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[19] Hong EJ, Lee DY, Kang HC, Kim YC, Shim MS, J. Ind. Eng. Chem., 63, 57 (2018)

[20] Wang H, Jiang H, Corbet C, de Mey S, Law K, Gevaert T, Feron O, De Ridder M, Cancer Lett., 450, 42 (2019)

[21] Hang W, Yin ZX, Liu G, Zeng Q, Shen XF, Sun QH, et al., Oncogene, 37, 3384 (2018)

[22] Venkatesan J, Alam MS, Hong EJ, Kim SJ, Shim MS, RSC Adv., 6, 79307 (2016)

[23] Liu Y, Chang Y, Yang C, Sang Z, Yang T, Ang W, Ye W, Wei Y, Gong C, Luo Y, Nanoscale, 6, 4325 (2014)

[24] Aodah A, Pavlik A, Karlage K, Myrdal PB, PLoS One, 11, e01517 (2016)

[25] Kobayashi H, Watanabe R, Choyke PL, Theranostics, 4, 81 (2014)

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[29] Lee EH, Kim JK, Lim JS, Lim SJ, Colloids Surf. B: Biointerfaces, 136, 305 (2015)

[30] Wang W, Liang G, Zhang W, Xing D, Hu X, Chem. Mater., 30, 3486 (2018)