Micro-grooved nerve guidance conduits combined with microfiber for rat sciatic nerve regeneration

Jin Jeon; Min Suk Lee; Juhan Lim; Sihyeon Park; Seong Min Kim; Dong-ik Kim; Giyoong Tae; Hee Seok Yang

Journal of Industrial and Engineering Chemistry, Vol.90, 214-223, October, 2020

DOI10.1016/j.jiec.2020.07.014 Export Citation

Micro-grooved nerve guidance conduits combined with microfiber for rat sciatic nerve regeneration

Jin Jeon^{1, 2}, Min Suk Lee^{1, 2}, Juhan Lim^{1, 2}, Sihyeon Park^{1, 2}, Seong Min Kim¹, Dong-ik Kim³, Giyoong Tae⁴, and Hee Seok Yang^{1, 2, †}

¹Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea
²Center for Bio-Medical Engineering Core-Facility, Dankook University, Cheonan 31116, Republic of Korea
³Division of Vascular Surgery, Samsung Medical Center, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea
⁴School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea

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In order to accelerate the regeneration of injured nerves, highly aligned structures that mimic native tissues are essential for nerve guidance conduits (NGC). In this study, a biodegradable porous micro- patterned NGCs were manufactured by use of capillary force lithography and salt leaching method, which filled as wrapped with aligned electrospun microfiber (PA-NGC) using. These combined NGC showed 5 μm aligned groove pattern on the interior wall and were filled with 4-5 μm align fibers for a fascicular structure to mimic the native nerve. The highly aligned structure has increased PC12 migration and morphological elongation compared with random microfiber. For the in vivo study, we bridged in the 10 mm sciatic nerve defect margins with NGC. The PA-NGC were compared to flat NGCs filled with random (FR-NGC) or aligned fiber (FA-NGC) and patterned NGCs filled with random fiber (PR-NGC). The PA-NGC showed as dramatically re-bridged neurofilament and higher sciatic function index, onset-to-peak of amplitude, muscle weight and fiber diameter compared to other groups after 8 weeks of implantation. In our results, the PA-NGC not only can serve as a design for functional NGC without growth factor but also can be used in clinical application for peripheral nerve regeneration.

Keywords:Aligned groove pattern;Electrospun microfiber;Nerve guidance conduit;Sciatic nerve generation

[References]

Mukhatyar V, Karumbaiah L, Yeh J, Bellamkonda R, Adv. Mater., 21, 3225 (2009)
Zhang D, Xu S, Wu S, Gao C, J. Mater. Chem. B, 6, 1226 (2018)
Sever M, Uyan I, Tekinay AB, Guler MO, Neu. Eng., 6, 181 (2016)
Daly W, Yao L, Zeugolis D, Windebank A, Pandit A, J. R. Soc. Interface, 9, 202 (2012)
Ribeiro-Resende VT, Koenig B, Nichterwitz S, Oberhoffner S, Schlosshauer B, Biomaterials, 30, 5251 (2009)
Chiono V, Tonda-Turo C, Prog. Neurobiol., 131, 87 (2015)
Sarker M, Naghieh S, McInnes AD, Schreyer DJ, Chen X, Biotechnol. J., 13, e17006 (2018)
Chew SY, Mi R, Hoke A, Leong KW, Adv. Funct. Mater., 17, 1288 (2007)
Xu X, Yee WC, Hwang PYK, Yu H, Wan ACA, Gao S, Boon KL, Mao HQ, Leong KW, Wang S, Biomaterials, 24, 2405 (2003)
Shen CC, Yang YC, Liu BS, Injury, 42, 830 (2011)
Sliow A, Ma Z, Gargiulo G, Mahns D, Mawad D, Breen P, Stoodley M, et al., Adv. Sci., 6, 180121 (2019)
Pinho AC, Fonseca AC, Serra AC, Santos JD, Coelho JF, Adv. Healthcare Mater., 5, 2732 (2016)
Yang K, Park E, Lee JS, Kim IS, Hong K, Park KI, Cho SW, Yang HS, Macromol. Biosci., 15, 1348 (2015)
Kim SM, Lee MS, Jeon J, Lee DH, Yang K, Cho SW, Han I, Yang HS, Macromol. Biosci., 18, 180029 (2018)
Lee MS, Lee DH, Jeon J, Oh SH, Yang HS, ACS Appl. Mater. Interfaces, 10, 38780 (2018)
Kim EC, Lee DY, Lee MH, Lee HJ, Kim KH, Leesungbok R, Ahn SJ, Park SJ, Yoon JH, Jee YJ, Lee SC, Lee SW, Tissue Eng. Regener. Med., 15, 615 (2018)
Peng SW, Li CW, Chiu IM, Wang GJ, Int. J. Nanomed., 12, 421 (2017)
Liu C, Kray J, Chan C, Tissue Eng. Regener. Med., 15, 351 (2018)
Sun B, Zhou Z, Li D, Wu T, Zheng H, Liu J, Wang G, Yu Y, Mo X, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 94, 190 (2019)
Mosahebi A, Woodward B, Wiberg M, Martin R, Terenghi G, Glia, 34, 8 (2001)
Wang CY, Zhang KH, Fan CY, Mo XM, Ruan HJ, Li FF, Acta Biomater., 7, 634 (2011)
Tonda-Turo C, Audisio C, Gnavi S, Chiono V, Gentile P, Raimondo S, Geuna S, Perroteau I, Ciardelli G, Adv. Eng. Mater., 13, B151 (2011)
Yekeen N, Padmanabhan E, Idris AK, Indust. Eng. Chem., 66, 45 (2018)
Hokmabad VR, Davaran S, Aghazadeh M, Alizadeh E, Salehi R, Ramazani A, Tissue Eng. Regener. Med., 15, 735 (2018)
Sionkowska A, Prog. Polym. Sci., 36, 1254 (2011)
Sell SA, Wolfe PS, Garg K, McCool JM, Rodriguez IA, Bowlin GL, Polymers, 2, 522 (2010)
Kohane DS, Langer R, Pediatr. Res., 63, 487 (2008)
Bellamkonda RV, Biomaterials, 27, 3515 (2006)
Clements IP, Kim YT, English AW, Lu X, Chung A, Bellamkonda RV, Biomaterials, 30, 3834 (2009)
Yucel D, Kose GT, Hasirci V, Biomaterials, 31, 1596 (2010)
Koh HS, Yong T, Teo WE, Chan CK, Puhaindran ME, Tan TC, Lim A, Lim BH, Ramakrishna S, J. Neural Eng., 7, 046003 (2010)
Yang HS, Ieronimakis N, Tsui JH, Kim HN, Suh KY, Reyes M, Kim DH, Biomaterials, 35, 1478 (2014)
Sarikcioglu L, Demirel BB, Utuk A, Folia Morphol., 68, 1 (2009)
Zeng CG, Xiong Y, Xie G, Dong P, Quan D, Tissue Eng. Part A, 20, 1038 (2014)
Kim DH, Mitchel JA, Bellamkonda RV, Annu. Rev. Biomed. Eng., 12, 203 (2010)
Chew SY, Mi R, Hoke A, Leong KW, Biomaterials, 29, 653 (2008)
Sun B, Zhou Z, Wu T, Chen W, Li D, Zheng H, El-Hamshary H, Al-Deyab SS, Mo X, Yu Y, ACS Appl. Mater. Interfaces, 9, 26684 (2017)
Madduri S, Papaloizos M, Gander B, Biomaterials, 31, 2323 (2010)
Yang K, Jung K, Ko E, Kim J, Park KI, Kim J, Cho SW, ACS Appl. Mater. Interfaces, 5, 10529 (2013)
Lee MR, Kwon KW, Jung H, Kim HN, Suh KY, Kim K, Kim KS, Biomaterials, 31, 4360 (2010)
Belkas JS, Shoichet MS, Midha R, Neurol. Res., 26, 151 (2004)
Yoon JK, Kim HN, Bhang SH, Shin JY, Han J, La WG, Jeong GJ, Kang S, Tissue Eng. Part A, 22, 654 (2016)

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