화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.38, No.5, 1066-1077, May, 2021
DOI10.1007/s11814-021-0775-7  Export Citation
Regenerated coenzyme-based preparation of bienzyme-polymer nanoconjugates and their applications for the synthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate
Yuan Lu, Hongqian Dai, Pengpeng Cheng, Hanbing Shi1, Lan Tang, Xingyuan Sun1, †, and Zhimin Ou†
  • College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang, China
  • 1The Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, Heilongjiang, China
E-mail:,
A modular approach was applied for the synthesis of bienzyme-polymer nanoconjugates (nano-BECs) (50-70 nm) consisting of two enzymes (carbonyl reductase and glucose dehydrogenase) conjugated within a single universal polymer scaffold. The amount of the product ethyl (R)-2-hydroxy-4-phenylbutyrate (R-HPBE) with nano-BECs as the catalyst was 533mM in a dibutyl phthalate-phosphate buffer (dibutyl phthalate-PB) biphasic system, while the amount of R-HPBE was 349mM using carbonyl reductase-poly(acrylic acid) as the catalyst, indicating that the nano- BECs have an advantage for coenzyme regeneration. Compared with a single aqueous phase, the substrate treatment capacity was improved at the interface of the dibutyl phthalate-PB biphasic system. Under the optimal reaction conditions (35 °C, 40 h, dibutyl phthalate-PB 1 : 1), nano-BECs can completely convert substrate into optically pure R-HPBE (enantiomeric excess (e.e.) >99.9%) in the organic-aqueous system.
Keywords:Asymmetric Reduction;Bienzyme-polymer Nanoconjugate;R-HPBE;Interfacial Biocatalysis;Coenzyme Regeneration
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