생체적 적용을 위한 전기전도성을 갖는 그래핀과 폴리카프로락톤 복합물질 전기방사 섬유형 필름

오준성; 이은정; Jun-Sung Oh; Eun-Jung Lee

Korean Journal of Materials Research, Vol.31, No.5, 278-285, May, 2021

DOI10.3740/MRSK.2021.31.5.278 Export Citation

생체적 적용을 위한 전기전도성을 갖는 그래핀과 폴리카프로락톤 복합물질 전기방사 섬유형 필름

Electroconductive Graphene-Combined Polycaprolactone Electrospun Films for Biological Applications

오준성, 이은정^†

단국대학교 나노바이오의과학과

Jun-Sung Oh, and Eun-Jung Lee^†

Department of Nano-Biomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Korea

E-mail:

This study produces electroconductive polycaprolactone (PCL)-based film with different amounts of graphene (G) through electrospinning, and the characteristics of the produced G/PCL composites are investigated. The G/PCL results are analyzed by comparing them with those obtained using pure PCL electrospun film as a control. The morphology of electrospun material is analyzed through scanning electron microscopy and transmission electron microscopy. Mechanical and electrical properties are also evaluated. Composites containing 1% graphene have the highest elongation rate, and 5% samples have the highest strength and elasticity. Graphene contents > 25% show electro-conductivity, which level improves with increase of graphene content. Biological characteristics of G/PCL composites are assessed through behavioral analysis of neural cell attachment and proliferation. Cell experiments reveal that compositions < 50% show slightly reduced cell viability. Moreover, graphene combinations facilitated cell proliferation compared to pure PCL. These results confirm that a 25 % G/PCL composition is best for application to systems that introduce external stimuli such as electric fields and electrodes to lead to synergistic efficiency of tissue regeneration.

Keywords:graphene;electroconductive;polycaprolactone;electrospinning;neural cell

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[2] Pereira IM, Marote A, Salgado AJ, Silva NA, Pharmaceuticals, 12 (2019)

[3] Prabhakaran MP, Venugopal J, Chan CK, Ramakrishna S, Nanotechnology, 19, 455102 (2008)

[4] Jahromi M, Razavi S, Bakhtiari A, J. Tissue Eng. Regener. Med., 13, 2077 (2019)

[5] Dvali L, Mackinnon S, Clin. Plast. Surg., 30, 203 (2003)

[6] Konofaos P, Halen JPV, J. Reconstr. Microsurg., 29, 149 (2013)

[7] Blacher S, Maquet V, Schils F, Martin D, Schoenen J, Moonen G, Jerome R, Pirard JP, Biomaterials, 24, 1033 (2003)

[8] Dhirendra SK, Rajesh V, Kirubanandan S, Curr. Top. Med. Chem., 8, 341 (2008)

[9] Wang S, Qiu J, Guo W, Yu X, Nie J, Zhang J, Zhang X, Liu Z, Mou X, Li L, Liu H, Adv. Biosyst., 1, 160004 (2017)

[10] Agarwal S, Jiang S, Encyclopedia of Polymeric Nanomaterials, p. 1, Springer, Berlin, Heidelberg (2014).

[11] Greiner A, Wendorff JH, Angew. Chem.-Int. Edit., 46, 5760 (2007)

[12] Duda S, Dreyer L, Behrens P, Wienecke S, Chakradeo T, Glasmacher B, Haastert-Talini K, BioMed Res. Int., 2014, 835269 (2014)

[13] Bhattarai DP, Aguilar LE, Park CH, Kim CS, Membranes, 8, 62 (2018)

[14] Thomson RC, Yaszemski MJ, Powers JM, Mikos AG, Biomaterials, 19, 1935 (1998)

[15] Wang F, Wu F, Huang Y, Compos. Appl. Sci. Manuf., 110, 126 (2018)

[16] Liu F, Wang X, Chen T, Zhang N, Wei Q, Tian J, Wang Y, Ma C, Lu Y, J. Adv. Res., 21, 91 (2020)

[17] Bai RG, Ninan N, Muthoosamy K, Manickam S, Prog. Mater. Sci., 91, 24 (2018)

[18] Lundin V, Herland A, Berggren M, Jager WEH, Teixeira AI, PLOs ONE, 6, e18624 (2011)

[19] Palmieri V, Sciandra F, Bozzi M, Spirito MD, Papi M, Front. Bioeng. Biotechnol., 8, 383 (2020)

[20] Savini G, Orefice RL, J. Mater. Res. Technol., 9, 16387 (2020)

[21] Hakamada M, Kuromura T, Chen Y, Kusuda H, Mabuchi M, Mater. Trans., 48, 32 (2007)

[22] Srikanth M, Asmatulu R, Cluff K, Yao L, ACS Omega, 4, 5044 (2019)

[23] Chen P, et al., Electrospun Materials for Tissue Engineering and Biomedical Applications, p. 299, Woodhead Publishing (2017).

[24] Ginestra P, J. Mech. Behav. Biomed. Mater., 100, 103387 (2019)