Optimization of phenolics and flavonoids extraction from the fruit of Empetrum nigrum var. japonicum from Jeju Island in South Korea

Jianyuan Zhang; Tai Gyu Lee

Journal of Industrial and Engineering Chemistry, Vol.98, 350-357, June, 2021

DOI10.1016/j.jiec.2021.03.031 Export Citation

Optimization of phenolics and flavonoids extraction from the fruit of Empetrum nigrum var. japonicum from Jeju Island in South Korea

Jianyuan Zhang, and Tai Gyu Lee^†

Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea

E-mail:

This study optimizes the extraction of phenolic and flavonoid compounds from Empetrum nigrum var. japonicum (crowberry) fruit using response surface methodology and evaluates the antioxidant activities of the extracts obtained. The optimized conditions predicted by Box-Behnken design were as followed: for total flavonoid content, extraction time is 43 min, extraction temperature is 52 °C, volume of solvent used for per gram of sample is 290 mL and ethanol concentration is 72%; the corresponding values for total phenolic content are 115 min, 58 °C, 670 mL, and 62%. The extracted phenolic and flavonoid contents under optimal conditions were 74.8 and 73.9 mg/g, respectively, which agreed with the prediction values of the model. In addition, crowberry extracts exhibited considerable scavenging ability to 2,2-dipheny- l,1-picrylhydrazyl and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) radicals, and the maximum scavenging ability reached 92.6% and 100.0%, respectively. This study is of great significance for the promoting the development of crowberry industry and the exploiting of natural antioxidant.

Keywords:Empetrum nigrum var. japonicum;Phenolic;Flavonoids;Extraction;Antioxidant;Response surface methodology

[References]

Bell JNB, Tallis JH, J. Ecol., 61, 289 (1973)
Park S, Lee EUN, Han S, Lee H, Lee S, J. Food. Biochem., 36, 675 (2012)
Xu YH, Wulan TY, Huang SL, Zhao M, Zhang SJ, J. Qiqihar Univ., 5, 61 (2019)
Zhang YL, Mei RQ, Liu Z, Wang GMLB, Chin. Tradit. Herb. Drugs, 45, 2293 (2014)
Li RM, Wang ZY, Kang YH, Wang R, Pharmacological Studies on Empetrum nigrum, Central South Pharmacy, p.175 2005.
Jurikova T, Mlcek J, Skrovnkova S. Balla S, Sochor J, Baron M, Sumczynski D, Molecules, 21, 1685 (2016)
Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum E, et al., J. Nutr., 132, 461 (2002)
Laaksonen O, Sandell M, Jarvinen R, Kallio H, Food Chem., 124, 1514 (2010)
Liu XY, Li F, Li AF, Sun AL, Liu RM, Fine Chem., 36, 664 (2018)
Galanakis CM, Foods, 9, 523 (2020)
Galanakis CM, Aldawoud TMS, Rizou M, Rowan NJ, Ibrahim SA, Foods, 9, 1701 (2020)
Panche AN, Diwan AD, Chandra SR, J. Nutr. Sci., 5, 1 (2016)
Chen S, Zeng Z, Hu N, Bai B, Wang H, Suo Y, Food Chem., 242, 1 (2017)
Hyun TK, Kim HC, Ko YJ, Kim JS, Saudi J. Biol. Sci., 23, 181 (2013)
Lacramioara O, Ciprian M, Iran. J. Public Health, 45, 263 (2016)
Ogawa K, Sakakibara H, Iwata R, Ishii T, Sato T, Goda T, Shimoi K, Kumazawa S, J. Agric. Food. Chem., 56, 4457 (2008)
Kim KC, Kang K, Piao M, Heo Y, Chae S, Kim G, Moon J, Yoo B, Hyun J, J. Toxicol. Environ. Health. Sci. A, 72, 1499 (2009)
Oka S, Kuniba R, Tsuboi N, Tsuchida S, Ushida K, Tomoshige S, Kuramochi K, Biosci. Biotechnol. Biochem., 84, 31 (2020)
Bezerra REMA, Santelli EP, Oliveira LS, Villar LAE, Talanta, 76, 965 (2008)
Liyana-Pathirana C, Shahidi F, Food Chem., 93, 47 (2004)
Bi W, Tian M, Row KH, J. Chromatogr. A, 1285, 22 (2013)
Wong KH, Li GQ, Li KM, Razmovski-Naumovski V, Chan K, Food Chem., 231, 231 (2017)
Belwal T, Dhyani P, Bhatt ID, Rawal RS, Pande V, Food Chem., 207, 115 (2016)
Uma DB, Wai HC, mustapha WW, Sains Malays, 39, 19 (2010)
Borges GDSC, Vieira FGK, Copetti C, Gonzaga LV, Fett R, Food Res. Int., 44, 708 (2011)
Jiang HL, Yang JL, Shi YP, Ultrason. Sonochem., 34, 325 (2017)
Galanakis C, Goulas V, Tsakona S, Manganaris G, Gekas V, Int. J. Food Prop., 16, 382 (2013)
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH, J. Food Drug Anal., 22, 296 (2014)
Sultana B, Anwar F, Ashraf M, Molecules, 14, 2167 (2009)
Garcia-Salas P, Morales-Soto A, Segura-Carretero A, Molecules, 15, 8813 (2010)
Thoo YY, Ho S, Liang J, Wai HC, Tan C, Food Chem., 120, 290 (2010)
He B, Zhang LL, Yue XY, Liang J, Jiang J, Gao XL, Yue PX, Food Chem., 204, 70 (2016)
Hyun TK, Kim MO, Lee H, Kim Y, Kim E, Kim JS, Food Chem., 141, 1947 (2013)
Brand-Williams W, Cuvelier ME, Berset C, J. Food. Sci. Technol., 28, 25 (1995)
Rawat S, Jugran A, Giri L, Bhatt ID, Rawal RS, Evidence-Based Complementary and Alternative Medicine, pp.512787 (2011).
Ameer K, Bae SW, Jo Y, Lee HG, Ameer A, Kwon JH, Food Chem., 229, 198 (2017)
Mao WH, Han LJ, Shi B, Sep. Sci. Technol., 43(3), 671 (2008)
Galanakis CM, Trends Food Sci. Technol., 79, 98 (2018)
Galanakis CM, Tsatalas P, Galanakis IM, Ind. Crop. Prod., 111, 30 (2018)
Kopustinskiene DM, Jakstas V, Savickas A, Bernatoniene J, Nutrients, 12, 457 (2020)
Galanakis CM, Trends Food Sci. Technol., 42, 44 (2015)

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