가용화제를 활용한 세라마이드 고함량의 수화 액정형 베시클 개발

박민선; 진병석; Min Seon Park; Byung Suk Jin

Applied Chemistry for Engineering, Vol.32, No.5, 541-546, October, 2021

DOI10.14478/ace.2021.1068 Export Citation

가용화제를 활용한 세라마이드 고함량의 수화 액정형 베시클 개발

Preparation of Hydrated Liquid Crystalline Vesicles Containing High Content of Ceramide using a Solubilizer

박민선, 진병석 ^†

동덕여자대학교 화학⋅화장품학부

Min Seon Park, and Byung Suk Jin^†

Division of Chemistry and Cosmetics, Dongduk Women’s University, Seoul 02748, Korea

E-mail:

초록

세라마이드와 인지질, 콜레스테롤 등과의 상호적 자가회합이 가능한 최적의 조성을 구성하여 세라마이드 고함량의 수화 액정형 베시클을 제조하였다. 혼합조성을 달리하여 다양한 베시클을 제조한 결과, 에지 액티베이터인 sodium deoxycholate (SDOC)와 가용화제인 PEG-60 hydrogenated castor oil (HCO 60)을 혼합하여 베시클을 만들었을 때, 가장 작은 나노 사이즈의 입자가 만들어지고, 베시클 분산액은 약산성이면서 가장 안정한 상태를 유지하였다. 또한 편광현미경과 열분석을 통해 SDOC와 HCO 60의 첨가가 세라마이드 등 지질 성분의 결정성 억제에 영향을 미치는 것으로 확인되었다. 베시클 분산액은 8주 동안의 고온의 장기 보관에서도 외관 및 점도의 변화없이 안정성이 그대로 유지되었다.

Hydrated liquid crystalline vesicles containing a high content of ceramide were prepared by constituting an optimal composition in which ceramides can be mutually self-associated with phospholipid and cholesterol. From the result of manufacturing various vesicles with different component composition, when the edge activator sodium deoxycholate (SDOC) and the solubilizer PEG-60 hydrogenated castor oil (HCO 60) were mixed to form vesicles, the smallest nano-sized particles were produced and the vesicle dispersion solution was weakly acidic and maintained the most stable state. In addition, it was confirmed through polarized light microscopy and thermal analysis that the addition of SDOC and HCO 60 had an effect on the inhibition of crystallinity of lipid components such as ceramide. The stability of the vesicle dispersion solution was maintained without change in appearance and viscosity even after long-term storage at high temperature for eight weeks.

Keywords:Ceramide;Phospholipid;Edge activator;Solubilizer;Vesicle

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Chang ALS, Chen SC, Osterberg L, Brandt S, Von Grote EC, Meckfessel MH, Geriatr. Nurs., 39, 24 (2018)
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Kahraman E, Kaykin M, Bektay HS, Gungor S, Cosmetics, 6, 52 (2019)
Rawlings A, Voegeli R, Cell Tissue Res., 351, 217 (2013)
Nakaune-Iijima A, Sugishima A, Omura G, Kitaoka, Kitaoka H, Tashiro T, Kageyama S, Hatta I, Chem. Phys. Lipids, 215, 56 (2018)
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Kim YJ, et al., Multi-layered lamellar granule and skin external application composition containing same, KR patent 10-1527580 (2015).
Su R, Yang L, Wang Y, Yu S, Guo Y, Deng J, Zhao Q, Jin X, Int J Nanomedicine, 12, 5203 (2017)
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Yilmaz E, Borchert HH, Eur J. Pharm. Biopharm., 60(1), 91 (2005)
Park MS, Choi JW, Lee SH, Jin BS, Appl. Chem. Eng., 31(5), 545 (2020)
Di Nardo A, Wertz P, Giannetti A, Seidenari S, Acta Derm. Venereol., 78, 27 (1998)
Lee SY, Lim YM, Jin BS, Appl. Chem. Eng., 28(6), 679 (2017)
Chen W, Su BG, Xing HB, Yang YW, Ren QL, Fluid Phase Equilib., 287(1), 1 (2009)
Khazanov E, Priev A, Shillemans JP, Barenholz Y, Langmuir, 24(13), 6965 (2008)
Hussain A, Singh S, Sharma D, Webster TJ, Shafaat K, Faruk A, Int J Nanomedicine, 17, 5087 (2017)
Jones MN, Adv. Colloid Interface Sci., 54, 93 (1995)
Lee EH, Kim A, Oh YK, Kim CK, Biomaterials, 26(2), 205 (2005)
Vemuri S, Rhodes CT, Pharmaceutica Acta Helvetiae., 70, 95 (1995)
Marsh D, Smith I, Biochim. Biophys. Acta, 298, 133 (1973)

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[1] Aoki M, Ogai K, Kobayashi M, Minematsu T, Nakatani T, Okuwa M, Sanada H, Sugama J, Skin Res. Technol., 25, 158 (1999)

[2] Chang ALS, Chen SC, Osterberg L, Brandt S, Von Grote EC, Meckfessel MH, Geriatr. Nurs., 39, 24 (2018)

[3] Fulmer AW, Kramer GJ, J. Invest. Derm., 86, 598 (1986)

[4] Tupker RA, Pinnagoda J, Coenraads PJ, Nater JP, Brit. J. of Derm, 123, 199 (1990).

[5] Kahraman E, Kaykin M, Bektay HS, Gungor S, Cosmetics, 6, 52 (2019)

[6] Rawlings A, Voegeli R, Cell Tissue Res., 351, 217 (2013)

[7] Nakaune-Iijima A, Sugishima A, Omura G, Kitaoka, Kitaoka H, Tashiro T, Kageyama S, Hatta I, Chem. Phys. Lipids, 215, 56 (2018)

[8] Nemes Z, Steinert PM, Exp. Mol. Med., 31, 5 (1999)

[9] Lee JH, Lee JB, Park MS, A cosmetic composition comprising a ceramide containing capsule, KR patent 10-2070557 (2020).

[10] Hong JH, Kim EJ, Macro-capsule containing ceramide and cosmetic composition comprising the same for moisturizing, KR patent 10-1826699 (2018).

[11] Kim YJ, et al., Multi-layered lamellar granule and skin external application composition containing same, KR patent 10-1527580 (2015).

[12] Su R, Yang L, Wang Y, Yu S, Guo Y, Deng J, Zhao Q, Jin X, Int J Nanomedicine, 12, 5203 (2017)

[13] Gebre-Mariam T, Wohlrab J, Tessema EN, Neubert RH, Ski. Pharmacol. Physiol., 30, 115 (2017)

[14] Tokudome Y, Saito Y, Sato F, Kikuchi M, Hinokitani T, Goto K, Colloids Surf. B: Biointerfaces, 73, 92 (2009)

[15] Vavrova K, Kovacık A, Opalka L, Eur. Pharm. J., 64, 1 (2017)

[16] Zhang Q, Flach CR, Mendelsohn R, Mao G, Pappas A, Mack MC, Walters RM, Southall MD, Clin. Cosmet. Investig. Dermatol., 8, 329 (2015)

[17] Cho WG, Kim KA, Jang SI, Cho BO, J. Soc. Cosmet. Sci. Kor., 44(1), 31 (2018)

[18] Yilmaz E, Borchert HH, Eur J. Pharm. Biopharm., 60(1), 91 (2005)

[19] Park MS, Choi JW, Lee SH, Jin BS, Appl. Chem. Eng., 31(5), 545 (2020)

[20] Di Nardo A, Wertz P, Giannetti A, Seidenari S, Acta Derm. Venereol., 78, 27 (1998)

[21] Lee SY, Lim YM, Jin BS, Appl. Chem. Eng., 28(6), 679 (2017)

[22] Chen W, Su BG, Xing HB, Yang YW, Ren QL, Fluid Phase Equilib., 287(1), 1 (2009)

[23] Khazanov E, Priev A, Shillemans JP, Barenholz Y, Langmuir, 24(13), 6965 (2008)

[24] Hussain A, Singh S, Sharma D, Webster TJ, Shafaat K, Faruk A, Int J Nanomedicine, 17, 5087 (2017)

[25] Jones MN, Adv. Colloid Interface Sci., 54, 93 (1995)

[26] Lee EH, Kim A, Oh YK, Kim CK, Biomaterials, 26(2), 205 (2005)

[27] Vemuri S, Rhodes CT, Pharmaceutica Acta Helvetiae., 70, 95 (1995)

[28] Marsh D, Smith I, Biochim. Biophys. Acta, 298, 133 (1973)