화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.29, No.10, 713-719, October, 2021
DOI10.1007/s13233-021-9081-9  Export Citation
Enhanced Silk Fibroin-Based Film Scaffold Using Curcumin for Corneal Endothelial Cell Regeneration
Do Kyung Kim1, 2, Sanghyuk Lee2, Joo Hee Choi3, Bo Sung Jung2, Ki Soo Kim2, Jeong Eun Song3, Rui L. Reis4, 5, and Gilson Khang3, †
  • 1Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Department of Bionanotechnology and Bio-Convergence Engineering, eonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea
  • 2CHA Graduate School of Medicine, 120 Hyeryong-ro, Pocheon, Gyeonggi 11160, Korea
  • 3Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Department of Bionanotechnology and Bio-Convergence Engineering, Jonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea
  • 43B’s Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal
  • 5ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimaraes, Portugal
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The high demand for transplantable corneas is unlikely to subside anytime soon as there is a persistent shortage of cadaveric cornea. The goal of this study was to fabricate film scaffolds with desirable properties of a corneal endothelial cells (CECs) carrier. We used biocompatible materials (curcumin (CC) and silk fibroin (SF)) to construct transparent film scaffolds for CEC regeneration. The film scaffolds were subjected to surface analysis, transparency, stiffness, thermal characterization, and hydrophilicity evaluation. Biological activity of CECs on CC/SF film was analyzed by MTT assay, morphological analysis, mRNA expression, and histological study. Our results showed that the CC/SF film scaffolds had enhanced roughness and transparency compared to the pristine SF film scaffold. The hydrophilicity of the CC/SF film scaffolds showed a topographical environment that encouraged cellular interaction and tissue integration. All the films showed stable thermal characters and an improved capacity for cell growth when a proper amount of CC was incorporated into the SF film scaffolds. The results indicate that a robust scaffold is suitable for CEC transplantation.
Keywords:cornea endothelial cells;curcumin;silk fibroin;silk;scaffold;cornea regeneration;film scaffold;tissue engineering
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