화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.32, No.6, 647-652, December, 2021
DOI10.14478/ace.2021.1084  Export Citation
당목향 뿌리 추출물의 인체 모유두세포 증식 및 모발 성장 관련 신호전달에 미치는 영향
Effect of Saussurea Lappa Root Extract on Proliferation and Hair Growth-related Signal Pathway in Human Hair Follicle Dermal Papilla Cells
최형철1, 2, 정노희1, †
  • 1충북대학교 공과대학 공업화학과
  • 2(주)에이치피앤씨
Hyoung-Chul Chio1, 2, and Noh-Hee Jeong1, †
  • 1Department of Engineering Chemistry, Chungbuk National University, Cheongju 28644, Korea
  • 2HP&C Ltd., Cheongju 28158, Korea
E-mail:
초록
본 연구에서는 에탄올과 노말헥산을 이용하여 당목향 뿌리 추출물을 제조하고, 인체 모유두세포의 세포증식 및 모발 성장 관련 신호전달에 미치는 영향을 평가하였다. 당목향 뿌리 추출물의 세포증식 효과는 MTT assay를 실시하였으며, ERK, Akt, Wnt/β-catenin 신호 경로, 5α-reductase의 발현을 western blot 분석을 통해 측정하였다. 당목향 뿌리 추출물은 인체 모유두세포의 증식을 유의하게 증가시켰고, 세포증식에 관여하는 ERK와 Akt의 인산화를 촉진하였으며, 당목향 뿌리 추출물에 의해 증가된 ERK, Akt 인산화 촉진과 세포증식은 MEK/ERK 억제제 PD98059와 PI3K/Akt 억제제LY294002에 의해 유의하게 감소되었다. 또한 당목향 뿌리 추출물은 GSK-3β (Ser9)의 인산화를 통한 β-catenin (Ser552, 675)의 인산화를 촉진함으로써 핵 내의 β-catenin 축적을 유도하였고, 5α-reductase type Ⅰ, Ⅱ의 활성을 억제 하였다. 종합적으로 당목향 뿌리 추출물은 모유두세포의 ERK, Akt 경로의 활성화를 통해 세포의 증식을 유도하며, β-catenin 신호 경로 활성화 및 5α-reductase 활성 억제를 통해 탈모 예방 및 모발 성장 효과를 나타냄으로써 헤어케어 제품의 소재로 응용가능성이 있음을 시사한다.
In this study, Saussurea Lappa roots were extracted using ethanol and n-hexane, and also the effects on proliferation of human hair dermal papilla cells and fibroblast and related signaling pathway were evaluated. 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay was conducted for cell proliferation effect of Saussurea Lappa root extract, and extracellular signal-related kinase (ERK), serine/threonine protein kinase (Akt), wingless-related integration site (Wnt)/β -catenin signaling pathway, and 5α-reductase expression through western blot analysis were measured. Saussurea Lappa root extract significantly increased human hair dermal papilla cells and propagation of fibroblast, promoted phosphorylation of ERK and Akt that get involved in cell proliferation. Additionally, Saussurea Lappa root extract significantly decreased promotion of Akt phosphorylation and cell proliferation by MEK/ERK inhibitor PD98059 and PI3K/Akt inhibitor LY294002. Also, Saussurea Lappa root extract induced intranuclear β-catenin accumulation by promoting phosphorylation of β-catenin (Ser552, 675) through phosphorylation of GSK-3β (Ser9), and suppressed activation of 5α-reductase type Ⅰ and Ⅱ. Overall, Saussurea Lappa root induces cell proliferation through vitalization of ERK and Akt route of human hair dermal papilla cells and fibroblast and apoptosis defense mechanism, and can be helpful in hair loss prevention and hair growth by vitalizing the β-catenin signaling pathway and inhibiting activation of 5α-reductase, which can be used as a potential hair care products.
Keywords:Saussurea Lappa root extract;Human hair dermal papilla cells;Cell proliferation;Signaling pathway;Hair care
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