A new type of polymeric LB film named "Reversed Duckweed" type LB film was fabricated by use of a new kind of amphiphilic polymer which was composed of a flexible hydrophilic epichlorohydrin-ethylene diamine crosslinking microgels and several hydrophobic stearic chains. It had highly ordered structure, including a well ordered layer structure and highly oriented hydrophobic alkyl chains in each layer, which was confirmed by low angle X-ray diffraction and polarized FTIR-ATR experiment.