Bacillus sphaericus (NCIM 2478) produced high levels of penicillin V acylase (100 U / g dry cells). when grown on cornsteep liquor - minerals medium at 25-degrees-C for 20 h. Supplementation of the medium either with 1 % (w/v) whole wheat bran or its aqueous extract brought about more than 70 % increase in the total enzyme activity. Moreover, deletion of Na2HPO4, MgSO4, CaCl2, FeSO4, CuSO4 and KCl from the medium affected neither growth nor enzyme production.