2-Cyano-2-phenyl-1-hexanol containing chiral quaternary carbon was resolved using a lipase. Among various reaction conditions, transesterification reaction (solvent; n-hexane, lipase; Candida cylindracea) gave high selectivity(20/80). Additionally, the selectivity could be improved(13/87) when pyridine(l%) was used as an additive. The ratios of resolved alcohols were determined by GC and H-1-NMR analysis using Mosher’s derivative.