Three types of multilayer air filter media were developed and evaluated. Two other existing filters were also used for comparison of filter performance. The pressure drop, the collection efficiency, and the dust-holding capacity of the tested filters were measured, and the internal structure of the filter media was analyzed by using a scanning electron microscope. The multilayer filters tested in this study are composed of pre-surface layer, surface layer, and substrate layer. Among those layers, the surface layer is mainly responsible for particle collection. As a test result, it was found that the thickness of a surface layer has the greatest effect on filtration performance of a multilayer air filter. Additionally, filtration velocity and electrostatic forces should be considered together as important parameters for multilayer air filter design.