Use of Long-chain Alcohol in Extraction and Purification of Lincomycin from Fermentation Broth

Bin Wu; Jiawen Zhu; Kui Chen; Lijun Ji; Jianguo Guo; Jianxi Zhao

Korean Journal of Chemical Engineering, Vol.20, No.2, 354-358, March, 2003

DOI10.1007/BF02697252 Export Citation

Use of Long-chain Alcohol in Extraction and Purification of Lincomycin from Fermentation Broth

Bin Wu^†, Jiawen Zhu, Kui Chen, Lijun Ji, Jianguo Guo, and Jianxi Zhao¹

Chemical Engineering Research Center, East China University of Science and Technology, Campus P.O. Box 542, 130 Meilong Road, Shanghai 200237, China
¹Yueyang Zhongxiang Kangshen Pharmaceutical Group Co. Ltd., Yueyang, Hunan Province, 41400, China

E-mail:

Low selectivity for lincomycin in butanol extraction process leads to relatively higher content of impurities. A novel process for extraction of lincomycin from fermentation broth was studied in this work. Mixture of n-octanol and n-decanol is used as extractant to replace n-butanol in extraction of lincomycin. Optimal operation conditions for the process have been studied. Due to higher extraction selectivity for lincomycin A by long-chain alcohol, content of impurity (lincomycin B) in the final product is much lower than that in product by butanol process. Furthermore, the practicability for combination of long-chain alcohol and butanol in purification of lincomycin was investigated.

Keywords:Extraction;Lincomycin;Long-chain Alcohol;Selectivity;Flocculation

[References]

Asmus PA, Landis JB, Vila CL, J. Chromatogr., 264, 241 (1983)
Han DH, Hong YK, Hong WH, Hong WH, Korean J. Chem. Eng., 17(5), 528 (2000)
Jariwala SL, U.S. Patent, 4,091,204 (1978)
Kim C, Ryu YW, Korean J. Chem. Eng., 10(4), 203 (1993)
Lu XZ, Cao SH, Pharmaceutical Ind., 17, 12 (1986)
Pratap VK, Kunwar M, Saxena H, Mishra SD, Clinician, 41, 158 (1977)
Wu ZC, Xu RF, Chen JY, Sol. Extr. Ion Exch., 15, 501 (1997)
Wu ZC, Zhang X, Xu RF, Chen JY, Sep. Sci. Technol., 33(2), 259 (1998)

[Referenced By]

[1] Asmus PA, Landis JB, Vila CL, J. Chromatogr., 264, 241 (1983)

[2] Han DH, Hong YK, Hong WH, Hong WH, Korean J. Chem. Eng., 17(5), 528 (2000)

[3] Jariwala SL, U.S. Patent, 4,091,204 (1978)

[4] Kim C, Ryu YW, Korean J. Chem. Eng., 10(4), 203 (1993)

[5] Lu XZ, Cao SH, Pharmaceutical Ind., 17, 12 (1986)

[6] Pratap VK, Kunwar M, Saxena H, Mishra SD, Clinician, 41, 158 (1977)

[7] Wu ZC, Xu RF, Chen JY, Sol. Extr. Ion Exch., 15, 501 (1997)

[8] Wu ZC, Zhang X, Xu RF, Chen JY, Sep. Sci. Technol., 33(2), 259 (1998)