화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.10, No.1, 16-23, January, 2004
Export Citation
Microbial degradation and enhanced bioremediation of polycyclic aromatic hydrocarbons
Seoug Han Woo, and Jong Moon Park†
  • Department of Chemical Engineering, School of Envrionment Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea
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Polycyclic aromatic hydrocarbons (PAHs) are found in various contaminated sites and attract great environmental concerns due to their toxic and carcinogenic properties. Although the microbial degradation is known as a major removal mechanism, the rate is relatively slow in natural environment. Therefore, acceleration of the intrinsic biodegradation rate is essential for the rapid and cost-effective removal of PAHs from the contaminated site. There are a number of factors affecting bioremediation of PAHs in a system consisting of soil, water, microorganisms, and pollutants. In this report, an overview of PAHs contamination, the important factors affecting their biodegradation, and recently developed technologies enhancing the bioremediation rate are discussed.
Keywords:bioaugmentation;bioavailability;biodegradation;bioremediation;PAH
  1. Sims RC, Overcash MR, Residue Rev., 88, 1 (1983)
  2. Juhasz AL, Naidu R, Intl. Biodet. Biodegrad., 45, 57 (2000) 
  3. Wilson SC, Jones KC, Environ. Pollut., 81, 229 (1993)
  4. Cerniglia CE, Biodegradation, 3, 351 (1992)
  5. Keith LH, Telliard WA, Environ. Sci. Technol., 13, 416 (1979)
  6. Mueller JG, Lantz SE, Blattmann BO, Chapman PJ, Environ. Sci. Technol., 25, 1045 (1991)
  7. Kastner M, Mahro B, Appl. Microbiol. Biotechnol., 44(5), 668 (1996)
  8. Sturman PJ, Stewart PS, Cunningham AB, Bouwer EJ, Wolfram JH, J. Contam. Hydrol., 19, 171 (1995)
  9. Carmichael LM, Pfaender FK, Biodegradation, 8, 1 (1997)
  10. Morgan P, Watkinson RJ, Crit. Rev. Biotechnol., 8, 305 (1989)
  11. USEPA, EPA/540/A5-91/009 (1993)
  12. Woo SH, Park JM, J. Chem. Technol. Biotechnol., 74(10), 937 (1999)
  13. Olson PE, Flechter JS, Philp PR, Environ. Sci. Pollut. R., 8, 243 (2001)
  14. Bockelmann A, Zamfirescu D, Ptak T, Grathwohl P, Teutsch G, J. Contam. Hydrol., 60, 97 (2003)
  15. Schwarzenbach RP, Gschwend PM, Imboden DM, Environmental Organic Chemistry, p. 621, John, Wiley & Sons, N.Y. (1993)
  16. Blumer M, Sci. Am., 234, 35 (1976)
  17. Gibson DT, Subramanian V, Microbial Degradation of Organic Compounds, D.T. Gibson Ed., pp. 181-252, Marcel Dekker, NY (1984)
  18. Smith MR, Biodegradation, 1, 191 (1990)
  19. Yen KM, Serdar CM, Crit. Rev. Microbiol., 15, 247 (1988)
  20. Kiyohara H, Nagao K, Nomi R, Agr. Biol. Chem. Tokyo, 40, 1075 (1976)
  21. Weissenfels WD, Beyer M, Klein J, Appl. Microbiol. Biotechnol., 32, 479 (1990)
  22. Aitken MD, Stringfellow WT, Nagel RD, Kazunga C, Chen SH, Can. J. Microbiol., 44, 743 (1998)
  23. Ye B, Siddiqi MA, Maccubbin AE, Kumar S, Sikka HC, Environ. Sci. Technol., 30, 136 (1996)
  24. Heitkamp MA, Freeman JP, Miller DW, Cerniglia CE, Appl. Environ. Microbiol., 54, 2549 (1988)
  25. Walter U, Beyer M, Klein J, Rehm HJ, Appl. Microbiol. Biotechnol., 34, 671 (1991)
  26. Mueller JG, Chapman PJ, Pritchard PH, Appl. Environ. Microbiol., 56, 1079 (1990)
  27. Kastner M, Breuerjammali M, Mahro B, Appl. Microbiol. Biotechnol., 41(2), 267 (1994)
  28. Barnsley EA, J. Bacteriol., 154, 113 (1983)
  29. Kiyohara H, Nagao K, J. Gen. Microbiol., 105, 69 (1978)
  30. Samanta SK, Chakraborti AK, Jain RK, Appl. Microbiol. Biotechnol., 53(1), 98 (1999)
  31. Guerin WF, Jones GE, Appl. Environ. Microbiol., 54, 937 (1988)
  32. Tian L, Ma P, Zhong JJ, Process Biochem., 37, 1431 (2002)
  33. Woo SH, Rittmann BE, Biodegradation, 11, 213 (2000)
  34. McCarty PL, In the Fifth Rudolf Research Conference, Rutgers. The State University, New Brunswick, NJ (1969)
  35. Roels JA, Biotechnol. Bioeng., 22, 2457 (1980)
  36. Stouthamer AH, VanVerseveld HW, Comprehensive Biotechnology, vol. I, M. Moo-Young Ed., pp. 215-238, Pergamon Press, NY (1985)
  37. VanBriesen JM, Rittmann BE, Biotechnol. Bioeng., 67(1), 35 (2000)
  38. Heijnen JJ, vanLoosdrecht MCM, Tijhuis L, Biotechnol. Bioeng., 40, 1139 (1992)
  39. vanBriesen JM, Biodegradation, 12, 265 (2001)
  40. Karickhoff SW, Brown DS, Scott TA, Water Res., 13, 241 (1979)
  41. Mihelcic JR, Luthy RG, Biodegradation, 4, 141 (1993)
  42. Guerin WF, Boyd SA, Appl. Environ. Microbiol., 58, 1142 (1992)
  43. Wodzinski RS, Coyle JE, Appl. Microbiol., 27, 1081 (1974)
  44. Manilal YB, alexander M, Appl. Microbiol. Biotechnol., 35, 401 (1991)
  45. Volkering F, Breure AM, Sterkenburg A, VanAndel JG, Appl. Microbiol. Biotechnol., 36, 548 (1992)
  46. Weissenfels WD, Klewer HJ, Langhoff J, Appl. Microbiol. Biotechnol., 36, 689 (1992)
  47. Ramaswami A, Ghoshal S, Luthy RG, Environ. Sci. Technol., 31, 2268 (1997)
  48. Li KY, Xu T, Cawly WA, Colapret JA, Bonner JS, Ernest A, Verramachaneni PB, Waste Manage., 14, 571 (1994)
  49. Zhang W, Maier WJ, Miller RM, Environ. Sci. Technol., 31, 2211 (1997)
  50. Calvillo YM, Alexander M, Appl. Microbiol. Biotechnol., 45(3), 383 (1996)
  51. Woo SH, Park JM, Rittmann BE, Biotechnol. Bioeng., 73(1), 12 (2001)
  52. Ehrhardt HM, Rehm HJ, Appl. Microbiol. Biotechnol., 21, 32 (1985)
  53. Chang HT, Rittmann BE, Environ. Sci. Technol., 21, 280 (1987)
  54. Carlucci AF, Shimp SL, Craven DB, FEMS Microbiol. Ecol., 38, 1 (1986) 
  55. Laor Y, Strom PF, Farmer WJ, Water Res., 33, 1719 (1999)
  56. Scheibenbogen K, Zytner RG, Lee H, Trevors JT, J. Chem. Technol. Biotechnol., 59(1), 53 (1994)
  57. Efroymson RA, Alexander M, Appl. Environ. Microbiol., 57, 1441 (1991)
  58. Harms H, Zehnder AJB, Appl. Environ. Microbiol., 61, 27 (1995)
  59. vanLoosdrecht MCM, Lyklema J, Norde W, Zehnder AJB, Microbiol. Rev., 54, 75 (1990)
  60. West CC, Harwell JF, Environ. Sci. Technol., 26, 2324 (1992)
  61. Volkering F, Breure AM, Rulkens WH, Biodegradation, 8, 401 (1998)
  62. Mulligan CN, Yong RN, Gibbs BF, Eng. Geol., 60, 371 (2001)
  63. Edwards DA, Luthy RG, Liu Z, Environ. Sci. Technol., 25, 127 (1991)
  64. Liu Z, Edwards DA, Luthy RG, Water Res., 26, 1337 (1992)
  65. Edwards DA, Adeel Z, Luthy RG, Environ. Sci. Technol., 28, 1550 (1994)
  66. Sun S, Inskeep WP, Boyd SA, Environ. Sci. Technol., 29, 903 (1995)
  67. Guha S, Jaffe PR, Environ. Sci. Technol., 30, 1382 (1996)
  68. Tiehm A, Appl. Environ. Microbiol., 60, 258 (1994)
  69. Volkering F, Breure AM, vanAndel JG, Rulkens WH, Appl. Environ. Microbiol., 61, 1699 (1995)
  70. Laha S, Luthy RG, Environ. Sci. Technol., 25, 1920 (1991)
  71. Bramwell DP, Laha S, Biodegradation, 11, 263 (2000)
  72. Cserhati T, Illes Z, Nemes I, Appl. Microbiol. Biotechnol., 35, 115 (1991)
  73. Neu TR, Microbiol. Rev., 60, 151 (1996)
  74. Chen P, Pickard MA, Gray MR, Biodegradation, 11, 341 (2000)
  75. Yuan SY, Wei SH, Chang BV, Chemosphere, 41, 1463 (2000)
  76. Hughes JB, Beckles DM, Chandra SD, Ward CH, J. Ind. Microbiol. Biotechnol., 18, 152 (1997)
  77. Venosa AD, Suidan MT, Wrenn BA, Strohmeier KL, Haines JR, Eberhart BL, King D, Holder E, Environ. Sci. Technol., 30, 1764 (1996)
  78. Churchill SA, Griffin RA, Jones LP, Chruchill PF, J. Environ. Qual., 24, 19 (1995)
  79. Ogunseitan OA, Delgado IL, Tsai YL, Olson BH, Appl. Environ. Microbiol., 57, 2873 (1991)
  80. Ogunseitan OA, Olson BH, Appl. Microbiol. Biotechnol., 38, 799 (1993)
  81. Chen SH, Aitken MD, Environ. Sci. Technol., 33, 435 (1999)
  82. Mahaffey WR, Gibson DT, Cerniglia CE, Appl. Environ. Microbiol, 54, 2415 (1988)
  83. Cidaria D, Deidda F, Bosetti A, Appl. Microbiol. Biotechnol., 41(6), 689 (1994)
  84. Marcoux J, Deziel E, Villemur R, Lepine F, Bisaillon JG, Beaudet R, J. Appl. Microbiol., 88, 655 (2000)
  85. Mueller JG, Resnick SM, Shelton ME, Pritchard PH, J. Ind. Microbiol., 10(95), 102 (1992)
  86. Lloyd-Jones G, Laurie AD, Hunter DWF, Fraser R, FEMS Microbiol. Ecol., 29, 69 (1999) 
  87. Widada J, Nojiri H, Omori T, Appl. Microbiol. Biotechnol., 60(1-2), 45 (2002)
  88. Lee SY, Lee SJ, Jung HT, J. Ind. Eng. Chem., 9(1), 9 (2003)
  89. Sayler GS, Layton AC, Annu. Rev. Microbiol., 44, 625 (1990) 
  90. Fleming JT, Yao WH, Sayler GS, Appl. Environ. Microbiol., 64, 3698 (1998)
  91. Herrick JB, Madsen EL, Batt CA, Ghiorse WC, Appl. Environ. Microbiol., 59, 687 (1993)
  92. Errampalli D, Okamura H, Lee H, Trevors JT, vanElsas JT, FEMS Microbiol. Ecol., 26, 181 (1998) 
  93. Sayler GS, Ripp S, Curr. Opin. Biotechnol., 11, 286 (2000)
  94. Ripp S, Nivens DE, Ahn Y, Werner C, Jarrel J, Easter JP, Cox CD, Burlage RS, Sayler GS, Environ. Sci. Technol., 34, 846 (2000)
  95. Drobnik J, Intl. Biodet. Biodegrad., 44, 3 (1999)