Monitoring of Bioventing Process for Diesel-Contaminated Soil by Dehydrogenase Activity, Microbial Counts and the Ratio of n-Alkane/Isoprenoid

Im-Gyu Byun; Hae-Uk Nam; Seung Koo Song; In-Seong Hwang; Tae-Ho Lee; Tae-Joo Park

Korean Journal of Chemical Engineering, Vol.22, No.6, 917-921, November, 2005

Im-Gyu Byun^†, Hae-Uk Nam¹, Seung Koo Song², In-Seong Hwang, Tae-Ho Lee³, and Tae-Joo Park

Department of Environmental Engineering, Pusan National University, Busan 609-735, Korea
¹POSCO Engineering & Construction Co., Ltd., Hwasung-Shi, Gyunggi-Do 445-810, Korea
²Department of Chemical Engineering, Pusan National University, Busan 609-735, Korea
³Department of Environmental Engineering, Andong National University, Andong 760-749, Korea

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Monitoring parameters were evaluated for a bioventing process that was designed to treat soils contaminated with diesel fuel. Statistical analyses were conducted to evaluate correlations between total petroleum hydrocarbon concentrations in the contaminated soil and physico-chemical parameters of soil such as microbial counts, dehydrogenase activity, and n-alkane/isoprenoid ratio. The correlation coefficients (r2) obtained showed that TPH concentrations in the bioventing system were strongly correlated with dehydrogenase activity (DHA), total heterotrophic bacterial count, and hydrocarbon utilizing bacterial count. Thus, it was concluded that these parameters could useful monitoring parameters for soils contaminated with diesel fuel.

Keywords:Bioventing;Monitoring;Total Petroleum Hydrocarbons (TPH);Dehydrogenase Activity;Microbial Counts;n-Alkane/Isoprenoid;Correlation

[References]

Allard AS, Neilson AH, Int. Biodeterior. Biodegrad., 39, 253 (1997)
Atlas RM, Bartha R, Adv. Microbial Ecol., 12, 287 (1992)
Balba MT, Al-Daher R, Al-Awadhi N, J. Microbiol. Methods, 32, 155 (1998)
Carter MR, Soil Sampling and Methods of Analysis, Lewis Publisher, U.S.A. (1993)
Dupont RR, Environ. Prog., 12, 45 (1993)
Hur JM, Park JA, Korean J. Chem. Eng., 20(2), 307 (2003)
Lesson A, Hichee RE, Principle and Practices of Bioventing: Volume I-bioventing Principles; Volume II-bioventing Design, National Risk Management Research Laboratory, U.S. EPA and U.S. Air Force Center for Environmental Excellence, Technology Transfer Division, Brooks AFB, Texas, 29 September (1995)
Margesin R, Schinner F, Appl. Microbiol. Biotechnol., 47(4), 462 (1997)
Margesin R, Zimmerbauer A, Schinner F, Chemosphere, 40, 339 (2000)
Nocentini M, Pinelli D, Fava F, Chemosphere, 41, 1115 (2000)
Noris RD, (Ed.), Handbook of Bioremediation, CRC press, Boca Raton, Florida (1994)
Page AL, Miller RH, Keeney DR, Methods of Soil Analysis, Part II, 2nd ed., ASA-SSSA, Wisconsin, 937-970 (1982)
Seklemova E, Pavlova A, Kovacheva K, Biodegradation, 12, 311 (2001)
Skujins J, "Extracellular Enzymes in Soil", CRC Crit. Rev. Microbiol., 4, 383 (1976)
US EPA, Bioventing Principle and Practice : Bioventing Principles, EPA, Washington, DC. (1995)
van Beelen PV, Doelman P, Chemosphere, 34, 455 (1997)

[Referenced By]

[1] Allard AS, Neilson AH, Int. Biodeterior. Biodegrad., 39, 253 (1997)

[2] Atlas RM, Bartha R, Adv. Microbial Ecol., 12, 287 (1992)

[3] Balba MT, Al-Daher R, Al-Awadhi N, J. Microbiol. Methods, 32, 155 (1998)

[4] Carter MR, Soil Sampling and Methods of Analysis, Lewis Publisher, U.S.A. (1993)

[5] Dupont RR, Environ. Prog., 12, 45 (1993)

[6] Hur JM, Park JA, Korean J. Chem. Eng., 20(2), 307 (2003)

[7] Lesson A, Hichee RE, Principle and Practices of Bioventing: Volume I-bioventing Principles; Volume II-bioventing Design, National Risk Management Research Laboratory, U.S. EPA and U.S. Air Force Center for Environmental Excellence, Technology Transfer Division, Brooks AFB, Texas, 29 September (1995)

[8] Margesin R, Schinner F, Appl. Microbiol. Biotechnol., 47(4), 462 (1997)

[9] Margesin R, Zimmerbauer A, Schinner F, Chemosphere, 40, 339 (2000)

[10] Nocentini M, Pinelli D, Fava F, Chemosphere, 41, 1115 (2000)

[11] Noris RD, (Ed.), Handbook of Bioremediation, CRC press, Boca Raton, Florida (1994)

[12] Page AL, Miller RH, Keeney DR, Methods of Soil Analysis, Part II, 2nd ed., ASA-SSSA, Wisconsin, 937-970 (1982)

[13] Seklemova E, Pavlova A, Kovacheva K, Biodegradation, 12, 311 (2001)

[14] Skujins J, "Extracellular Enzymes in Soil", CRC Crit. Rev. Microbiol., 4, 383 (1976)

[15] US EPA, Bioventing Principle and Practice : Bioventing Principles, EPA, Washington, DC. (1995)

[16] van Beelen PV, Doelman P, Chemosphere, 34, 455 (1997)