황련유래 단백질이 함유된 나노리포좀의 제조 및 특성

오승룡; 이상봉; 조계민; 최문재; 진병석; 한용문; 이영무; 심진기; Seng Ryong Oh; Sang Bong Lee; Kye Min Cho; Moon Jae Choi; Byung Suk Jin; Yong Moon Han; Young Moo Lee; Jin Kie Shim

Journal of the Korean Industrial and Engineering Chemistry, Vol.17, No.1, 52-57, February, 2006

Export Citation

황련유래 단백질이 함유된 나노리포좀의 제조 및 특성

Preparation and Characterizatino of Nano-sized Liposome Containing Proteins Derived from Coptidis rhizoma

오승룡, 이상봉 , 조계민, 최문재¹, 진병석 ², 한용문³, 이영무 ⁴, 심진기^†

한국생산기술연구원
¹(주)네비온
²동덕여자대학교 응용화학과
³동덕여자대학교 약학대학
⁴한양대학교 화학공학과

Seng Ryong Oh, Sang Bong Lee , Kye Min Cho, Moon Jae Choi¹, Byung Suk Jin², Yong Moon Han³, Young Moo Lee⁴, and Jin Kie Shim^†

Korea Institute of Industrial Technology, Chongwang, Shihung, Gyeonggi-do 101-1BL, Korea
¹Nabion Corporation, Sangdaewon-dong, Sungnam, Gyeonggi-do 462-120, Korea
²Department of Applied Chemistry, Dongduk Women's University, Seoul 136-714, Korea
³College of Pharmacy, Dongduk Women's University, Seoul 136-714, Korea
⁴School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Korea

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초록

천연 향진균 물질인 황련(Coptidis Rhizoma)은 감염성 피부염을 유발시키는 주 원인균인 Cadida albicans에 항세균 작용이 있다고 알려져 있다. 본 연구에서는 황련유래 단백질(Proteins derived from Coptidis Rhizoma, CRP)의 유효 성분을 추출하여 안정성 도모 및 피부 세포 내에 안정하게 흡수시키는 물질 전달체로서 리포좀을 제조하였다. 황련유래 단백질을 함유한 리포좀 입자의 평균 크기는 187 nm, 표면전하는 3.337 mV이며 포집효율은 33%이었다. 온도, 저장시간에 따른 CRP의 방출 거동을 확인하였으며, 리포좀의 안정성을 증가시키기 위하여 리포좀을 poly(vinyl alcohol) (PVA)로 코팅하였다. 코팅된 리포좀은 코팅 안된 리포좀에 비하여 낮은 방출율을 나타내었다. 또한, 리포좀에 함유된 CRP의 항캔디다 효과는 50 ℃ 또는 자외선(UV)으로 24 h 처리한 후에도 지속됨을 확인하였다.

Coptidis Rhizoma, an antimicrobial agent from natural source, is known to have the antiviral effect on the Candida albicans that causes the infectious dermatitis. The valuable protein was extracted from the Coptidis Rhizoma, To prevent denaturalization from external stimulus and improve adsorption onto the skin, the nano-sized liposomes were prepared as a carrier. The CPR-containing liposomes showed an average diameter of 187 nm, surface charge of 3.337 mV and 33% encapsulation efficiencyl. Therelease behavior of CRP from the liposome was investigated with various temperature and releasing time. The PVA solution was coated on the surface of liposome to improve the stability. The coated liposome showed slow release behavior in comparison with the non-coated liposome. The CRP in the liposome maintained the effect on the Candida albicans after treating it at 50 and with ultraviolet for 24 h.

Keywords:coptidis rhizoma;candida albicans;liposome;surface coating

[References]

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Guo J, Ping Q, Jiang G, Huang L, Tong Y, Int. J. Pharm., 260, 167 (2003)
Ruel-Gariepy E, Leclair G, Hildgen P, Gupta A, Leroux JC, J. Control. Release, 82, 373 (2002)
Krieg RC, Dong Y, Schwamborn K, Knuechel R, J. Biochem. Biophys. Methods, 65, 13 (2005)
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[1] Weinberger M, Leibovici L, Perez S, Samra Z, Ostfeld I, Levi I Bash E, Turner D, Goldschmied-Reouven A, Regev-Yochay G, Pitlik SD, Keller N, J. Hosp. Infec., in press

[2] Viejo-Diaz M Andres MT, Fierro JF, FEMS Immunolog. Medi. Microbiology., 42, 181 (2004)

[3] Mohammedi I, Thiebaut A, Piens MA, Argaud L, Martin O, Robert D, J. Infec., 1 (2004)

[4] Franzblau SG, Cross C, J. Ethnopharmacol., 15, 279 (1986)

[5] Miura N, Yamamoto M, Ueki T, Kitani T, Fukuda K, Komatsu Y, Biochem. Pharmacol., 53, 1315 (1997)

[6] Freile ML, Giannini F, Pucci G, Sturniolo A, Rodero L, Pucci O Balzareti V, Enriz RC, Fitoterapia., 74, 702 (2003)

[7] Kim HK, Lee JH, Shin JK, Han YM, J. Pharm. Soc. Korea., 49, 323 (2005)

[8] Feng SS, Huang GF, J. Control. Release, 71, 53 (2001)

[9] Jin BS, Han YM, Lee KH, Choi MJ, J. Korean Ind. Eng. Chem., 15(2), 205 (2004)

[10] Feng SS, Ruan G, Li QT, Biomaterials, 25, 5181 (2004)

[11] Touitou E, Dayan N, Bergelson L, Godin B, Eliaz M, J. Control. Release, 65, 403 (2000)

[12] Godin B, Touitou E, J. Control. Release, 94, 365 (2004)

[13] Barry BW, Eur. J. Pharm. Sci., 14, 101 (2001)

[14] Bouwstra JA, Honeywell-Nguyen PL, Adv. Drug Deliv. Rev., 54, S41 (2002)

[15] Iga K, Ogawa Y, Toguchi H, Pharm. Res., 9, 658 (1992)

[16] Ono A, Yamaguchi M, Horikoshi I, Shintani T, Ueno M, Biol. Pharm. Bull., 17, 166 (1994)

[17] Kono K, Hayashi H, Takagishi T, J. Control. Release, 30, 69 (1994)

[18] Kono K, Henmi A, Yamashita H, Hayashi H, Takagishi T, J. Control. Release, 59, 63 (1999)

[19] Kono K, Yoshino K, Takagishi T, J. Control. Release, 80, 32 (2002)

[20] Takeuchi H, Kjima H, Toyoda T, Yamamoto H, Hino T, Kawashima Y, Eur. J. Pharm. Biopharm., 48, 123 (1999)

[21] Takeuchi H, Kojima H, Yamamoto H, Kawashima Y, J. Control. Release, 68, 195 (2000)

[22] Takeuchi H, Yamamoto H, Toyoda T, Toyobuku H, Int. J. Pharm., 164, 103 (1998)

[23] Guo J, Ping Q, Jiang G, Huang L, Tong Y, Int. J. Pharm., 260, 167 (2003)

[24] Ruel-Gariepy E, Leclair G, Hildgen P, Gupta A, Leroux JC, J. Control. Release, 82, 373 (2002)

[25] Krieg RC, Dong Y, Schwamborn K, Knuechel R, J. Biochem. Biophys. Methods, 65, 13 (2005)

[26] Determan AS, Trewyn BG, Lin VSY, Nilsen-Hamilton M, Narasimhan B, J. Control. Release, 100, 97 (2004)

[27] Remun an-Lopez C, Lorenzo-Lamosa ML, Vila-Jato JL, Alonso MJ, Eur. J. Pharm. Biopharm., 45, 49 (1998)

[28] Cirli OO, Hasirci V, J. Control. Release, 96, 85 (2004)

[29] Joshi BN, Sainani MN, Bastawade KB, Gupta VS, Ranjekar PK, Biochem. Biophys. Res. Commun., 246, 382 (1998)