Macromolecular Research, Vol.16, No.6, 544-548, August, 2008
Temperature Controllable HPLC Column for Preparative Fractionation of Polymers
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An HPLC column with a self-contained temperature control device was constructed for preparative temperature programmed interaction chromatography. Two Peltier plates were attached to a large bore column (120×22mm i.d.) and the column temperature was controlled by PID mode feed back control. At a flow rate of 1.5 mL/min, the column temperature could be increased and decreased at a rate as high as 50 ℃/min and 10 ℃/min, respectively, which is much faster than using a column jacket and bath/circulator. The rapid heating and cooling rates allows a high repetition rate of chromatographic fractionation. The performance of the temperature controllable column was demonstrated successfully by the fractionation of homo-polymer precursors from diblock copolymers.
Keywords:polymer fractionation;HPLC column;temperature control;Peltier device;temperature gradient interaction chromatography
- Rabek JF, Experimental Methods in Polymer Chemistry, Wiley, New York (1980)
- Mori S, Barth HG, Size Exclusion Chromatography, Springer Verlag, New York (1999)
- Cho H, Park S, Ree M, Chang T, Jung JC, Zin WC, Macromol. Res., 14(3), 383 (2006)
- Glockner G, Gradient HPLC of Copolymers and Chromato-Graphic Cross-fractionation, Springer Verlag, Berlin (1992)
- Pasch H, Trathnigg B, HPLC of Polymers, Springer-Verlag, Berlin (1997)
- Chang T, Adv. Polym. Sci., 163, 1 (2003)
- Chang T, J. Polym. Sci. Part B: Polym. Phys., 43, 1591 (2005)
- Schoenmakers P, Fitzpatrick F, Grothey R, J. Chromatogr. A, 965, 93 (2002)
- Chang T, Lee HC, Lee W, Park S, Ko C, Macromol. Chem. Phys., 200, 2188 (1999)
- Lee HC, Lee W, Chang T, Korea Polym. J., 4(2), 160 (1996)
- Ryu J, Chang T, Anal. Chem., 77, 6347 (2005)
- Jones BA, J. Liq. Chromatogr. Rel. Technol., 27, 1331 (2004)
- Lee HC, Chang TY, Polymer, 37(25), 5747 (1996)
- Lee W, Lee H, Cha J, Chang T, Hanley KJ, Lodge TP, Macromolecules, 33(14), 5111 (2000)
- Lee HC, Chang TH, Harville S, Mays JW, Macromolecules, 31(3), 690 (1998)
- Cho D, Park S, Chang T, Ute K, Fukuda I, Kitayama T, Anal. Chem., 74, 1928 (2002)
- Park I, Park S, Cho DY, Chang TY, Kim E, Lee KY, Kim YJ, Macromolecules, 36(22), 8539 (2003)
- Park S, Cho D, Im K, Chang T, Uhrig D, Mays JW, Macromolecules, 36(15), 5834 (2003)
- Im K, Park S, Cho D, Chang T, Lee K, Choi N, Anal. Chem., 76, 2638 (2004)
- Park S, Park I, Chang T, Ryu CY, J. Am. Chem. Soc., 126(29), 8906 (2004)
- Park S, Chang T, Macromolecules, 39(9), 3466 (2006)
- Park S, Ko C, Choi H, Kwon K, Chang T, J. Chromatogr. A, 1123, 22 (2006)
- Im K, Park HW, Kim Y, Chung BH, Ree M, Chang TH, Anal. Chem., 79, 1067 (2007)
- Santangelo PG, Roland CM, Chang T, Cho D, Roovers J, Macromolecules, 34(26), 9002 (2001)
- Park S, Kwon K, Cho D, Lee B, Ree M, Chang T, Macromolecules, 36(12), 4662 (2003)
- Welsch T, Schmid M, Kutter J, Kalman A, J. Chromatogr. A, 728, 299 (1996)
- Kwon K, Lee W, Cho D, Chang T, Korea Polym. J., 7(5), 321 (1999)
- Lee W, Cho DY, Chang TY, Hanley KJ, Lodge TP, Macromolecules, 34(7), 2353 (2001)
- Hsieh HL, Quirk RP, Anionic Polymerization ; Principles and Practical Applications, Marcel Dekker, New York (1996)
- Hadjichristidis N, Iatrou H, Pitsikalis M, Mays J, Prog. Polym. Sci, 31, 1068 (2006)
- Hirao A, Tsunoda Y, Matsuo A, Sugiyama K, Watanabe T, Macromol. Res., 14(3), 272 (2006)
- Chung BH, Park S, Chang TY, Macromolecules, 38(14), 6122 (2005)
- Park S, Cho D, Ryu J, Kwon K, Lee W, Chang T, Macromolecules, 35(15), 5974 (2002)
- Park I, Park S, Park HW, Chang T, Yang HC, Ryu CY, Macromolecules, 39(1), 315 (2006)
- Hwang SW, Kim E, Shin C, Kim JH, Ryu DY, Macromolecules, 40(22), 8066 (2007)
- Nguyen SH, Berek D, Chiantore O, Polymer, 39(21), 5127 (1998)
- Lazzari M, Janco M, Kitayama T, Hatada K, Macromol. Rapid Commun., 24(17), 1019 (2003)