화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.26, No.4, 1084-1089, July, 2009
DOI10.1007/s11814-009-0180-0  Export Citation
Production of hydrocortisone by Absidia coerulea in moderate pressure bioconversion system
Shi Ru Jia†, and Jian Dong Cui1
  • Key Laboratory of Industry Microbiology, Ministry of Education, Tianjin University of Science and Technology, 29 Thirteenth Street, Tai Da Development Area, Tianjin 300457, P. R. China
  • 1Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang 050018, P. R. China
E-mail:
The effects of moderate pressure (0.1-2.5 MPa) on viability, cell membrane permeability and catalyzing activity of Absidia coerulea for RSA were investigated. A new method for improving the production of Hydrocortisone (HC) from 17α-hydroxypregn-4-en-3, 20-dione-21-acetate by Absidia coerulea in moderate pressure was developed. The results showed that the morphology of Abasidia coerulea mycelium was changed in moderate pressure, Absidia coerulea mycelium seemed to be loosed, and cell membrane permeability of Abasidia coerulea mycelium was improved. However, the viability of Abasidia coerulea mycelium could keep high level. Moreover, the yield of HC was improved over 1.25-fold as compared with that of the control (untreated cells), to give the yield of HC as 350 mg/l, when the Abasidia coerulea mycelium was treated with 0.5Mpa the atmosphere as the pressure media. Especially, the production of HC with atmosphere as the pressure media (0.5 MPa) could be increased by the addition of H2O2 (60 mmol/l); the relative yield of HC in moderate pressure was enriched by over 4.5% in comparison with the control. The major composition of bioconverted mixture was reduced. It was indicated that the new approach (moderate pressure) obtained in this work possessed a high potential for the industrial production of HC.
Keywords:Abasidia coerulea;Hydrocortisone;Permeability;Viability;Moderate Pressure;Biotransformation
  1. Mohammad AF, Mojtaba TY, Gholamreza SAZ, Steroids, 67, 869 (2002)
  2. Porter RBR, Gallimore WA, Reese PB, Steroids, 64, 770 (1999)
  3. Anmad S, Garg SK, Johri BN, Biotech. Adv., 10, 1 (1992)
  4. Dordick JS, Enzyme. Microb. Technol., 11, 194 (1989)
  5. Abramov S, Aharonowitz Y, Harnik M, Enzyme. Microb. Technol., 12, 982 (1990)
  6. Wang J, Chen CZ, Li BA, Zhang JH, Yu YT, Enzyme Microb. Technol., 22(5), 368 (1998)
  7. Jia SR, Li B, Park YS, J. Fermen. Biosci., 2, 191 (1996)
  8. Coelho MAZ, Belo I, Pinheiro R, Amaral AL, Mota M, Coutinho JAP, Ferreira EC, Appl. Microbiol. Biotechnol., 66(3), 318 (2004)
  9. Manas P, Mackey BM, Appl. Environ. Microbiol., 70, 1545 (2004)
  10. Abe F, Kato C, Horikoshi K, Trends. Microbiol., 11, 447 (1999)
  11. Bothun GD, Knutson BL, Berberich JA, Strobel HJ, Nokes SE, Appl. Microbiol. Biotechnol., 65(2), 149 (2004)
  12. Bartlett DH, Kato C, Horikoshi K, Res. Microbiol., 146, 697 (1995)
  13. Sharma A, Scott JH, Cody GD, Science, 295, 1514 (2002)
  14. Kato M, Hayashi R, Tsuda T, Eur. J. Biochem., 269, 110 (2002)
  15. Perrier-Cornet JM, Hayert M, Gervais P, J. Appl. Microbiol., 87, 1 (1999)
  16. Sato S, Mukataka S, Kataoka H, J. Ferment. Technol., 59, 221 (1981)
  17. Matsui T, Shinzato N, Ypkpta H, Process Biochem., 41, 920 (2006)
  18. Cui JD, Li Y, Korean J. Chem. Eng., (In print) (2009)
  19. Byun IG, Nam HU, Song SK, Hwang IS, Lee TH, Park TJ, Korean J. Chem. Eng., 22(6), 917 (2005)
  20. Rhim JA, Yoon JH, Korean J. Chem. Eng., 22(2), 201 (2005)