Application of gas anti-solvent process to the recovery of andrographolide from Andrographis paniculatanees

Manop Charoenchaitrakool; Wuttichai Trisilanun; Penjit Srinopakhun

Korean Journal of Chemical Engineering, Vol.27, No.3, 950-954, March, 2010

DOI10.1007/s11814-010-0128-4 Export Citation

Application of gas anti-solvent process to the recovery of andrographolide from Andrographis paniculatanees

Manop Charoenchaitrakool^†, Wuttichai Trisilanun, and Penjit Srinopakhun

Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand

E-mail:

The gas anti-solvent (GAS) process was employed to extract andrographolide, which is the active ingredient found in Andrographis Paniculatanees, using carbon dioxide as an anti-solvent. The effects of temperature, flow rate and solvent type on the extraction recovery, particle size and morphology were investigated in this study. The experiments were conducted at the temperature ranging from 25-45 ℃, carbon dioxide flow rate of 1-15 mL/min, and various types of organic solvents (methanol, ethanol, acetone and N,N-dimethylformamide). The extracted product was analyzed using high performance liquid chromatography (HPLC). The highest extraction yield was found to be 1.24 g andrographolide per 100 g of A. paniculata when using acetone as a solvent, carbon dioxide flow rate of 5 mL/min and the temperature of 35 ℃. It was also found that no significant change in size or morphology of the precipitates was observed when changing temperature, carbon dioxide flow rate and solvents.

Keywords:Dense Gas;GAS Process;Andrographolide;Andrographis paniculatanees;Carbon Dioxide

[References]

McHugh MA, Krukonis VJ, Supercritical fluid extraction: Principles and practice, Butterworths, Boston (1986)
Kang KY, Ahn DH, Wilkinson GT, Chun BS, Korean J. Chem. Eng., 22(3), 399 (2005)
Sangarunlert W, Piumsomboon P, Ngamprasertsith S, Korean J. Chem. Eng., 24(6), 936 (2007)
Gallagher PM, Coffey MP, Krukonis VJ, Klasutis N, Gas antisolvent recrystallization: new process to recrystallize compounds insoluble in SCF. In supercritical fluid science and technology, Jonhston KP, Penninger JML (Eds.), ACS Symposium Series 406, American Chemical Society, Washington, DC, 334 (1989)
Chang CJ, Randolph AD, AIChE J., 36, 939 (1990)
Dixon DJ, Johnston KP, AIChE J., 37, 1441 (1991)
Yeo SD, Debenedetti PG, Radosz M, Schmidt HW, Macromolecules, 26, 6207 (1993)
Yeo SD, Lim GB, Debenedetti PG, Bernstein H, Biotechnol. Bioeng., 41, 341 (1993)
Bleich J, Mueller BW, Wassmuss W, Int. J. Pharm., 97, 111 (1993)
Park CI, Shin MS, Kim H, Korean J. Chem. Eng., 25(3), 581 (2008)
Trisilanun W, Charoenchaitrakool M, Extraction of active ingredients in andrographis paniculatanees using gas anti-solvent process (GAS), In the Proceedings of the 15th Thailand Chemical Engineering and Applied Chemistry Conference, Pattaya, Thailand, October 27-28 (2005)
Calabrese C, Berman SH, Babish JG, Ma X, Shinto L, Dorr M, Wells K, Wenner CA, Standish LJ, Phytother. Res., 14, 333 (2000)
Kumoro AC, Hasan M, Chin. J. Chem. Eng., 15(6), 877 (2007)
Ge FH, Lin XX, Huan XF, Shi Q, Liang B, Li J, Zhong G, J. Chinese Med. Mater., 25, 101 (2002)
Warwick B, Dehghani F, Foster NR, Biffin JR, Regtop HL, Ind. Eng. Chem. Res., 41(8), 1993 (2002)
Chen KX, Zhang XY, Pan J, Yin WH, J. Cryst. Growth, 274(1-2), 226 (2005)
Thiering R, Dehghani F, Dillow A, Foster NR, J. Chem. Technol. Biotechnol., 75(1), 29 (2000)

[Referenced By]

[1] McHugh MA, Krukonis VJ, Supercritical fluid extraction: Principles and practice, Butterworths, Boston (1986)

[2] Kang KY, Ahn DH, Wilkinson GT, Chun BS, Korean J. Chem. Eng., 22(3), 399 (2005)

[3] Sangarunlert W, Piumsomboon P, Ngamprasertsith S, Korean J. Chem. Eng., 24(6), 936 (2007)

[4] Gallagher PM, Coffey MP, Krukonis VJ, Klasutis N, Gas antisolvent recrystallization: new process to recrystallize compounds insoluble in SCF. In supercritical fluid science and technology, Jonhston KP, Penninger JML (Eds.), ACS Symposium Series 406, American Chemical Society, Washington, DC, 334 (1989)

[5] Chang CJ, Randolph AD, AIChE J., 36, 939 (1990)

[6] Dixon DJ, Johnston KP, AIChE J., 37, 1441 (1991)

[7] Yeo SD, Debenedetti PG, Radosz M, Schmidt HW, Macromolecules, 26, 6207 (1993)

[8] Yeo SD, Lim GB, Debenedetti PG, Bernstein H, Biotechnol. Bioeng., 41, 341 (1993)

[9] Bleich J, Mueller BW, Wassmuss W, Int. J. Pharm., 97, 111 (1993)

[10] Park CI, Shin MS, Kim H, Korean J. Chem. Eng., 25(3), 581 (2008)

[11] Trisilanun W, Charoenchaitrakool M, Extraction of active ingredients in andrographis paniculatanees using gas anti-solvent process (GAS), In the Proceedings of the 15th Thailand Chemical Engineering and Applied Chemistry Conference, Pattaya, Thailand, October 27-28 (2005)

[12] Calabrese C, Berman SH, Babish JG, Ma X, Shinto L, Dorr M, Wells K, Wenner CA, Standish LJ, Phytother. Res., 14, 333 (2000)

[13] Kumoro AC, Hasan M, Chin. J. Chem. Eng., 15(6), 877 (2007)

[14] Ge FH, Lin XX, Huan XF, Shi Q, Liang B, Li J, Zhong G, J. Chinese Med. Mater., 25, 101 (2002)

[15] Warwick B, Dehghani F, Foster NR, Biffin JR, Regtop HL, Ind. Eng. Chem. Res., 41(8), 1993 (2002)

[16] Chen KX, Zhang XY, Pan J, Yin WH, J. Cryst. Growth, 274(1-2), 226 (2005)

[17] Thiering R, Dehghani F, Dillow A, Foster NR, J. Chem. Technol. Biotechnol., 75(1), 29 (2000)