Amperometric study of hydrogen peroxide biosensor with butadiene rubber as immobilization matrix

Beom-Gyu Lee; Keun-Bae Rhyu; Kil-Joong Yoon

Journal of Industrial and Engineering Chemistry, Vol.16, No.3, 340-343, May, 2010

DOI10.1016/j.jiec.2010.01.016 Export Citation

Amperometric study of hydrogen peroxide biosensor with butadiene rubber as immobilization matrix

Beom-Gyu Lee, Keun-Bae Rhyu¹, and Kil-Joong Yoon^{1, †}

Department of Chemistry, Chosun University, Kwangju 501-759, Republic of Korea
¹Division of Applied Sciences, Cheongju University, Cheongju 360-764, Republic of Korea

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A carbon paste electrode bound by butadiene rubber has been newly constructed and its electrochemical properties have been investigated to test the practicability of the enzyme electrode. The binder of carbon powder was butadiene rubber dissolved in toluene and ground cabbage tissue was embedded in the matrix as an enzyme source. The electrode, which showed a mechanical robustness after volatilization of solvent, displayed good catalytic power (detection limit = 2.5 × 10^(-5) M, S/N = 2) and electrochemically irreversible characteristics. Its symmetry factor and the exchange current density of the electrode used were 0.23 and 1.71 × 10^(-3) A cm^(-2), respectively.

Keywords:Butadiene rubber;Biosensor;Hydrogen peroxide;Peroxidase;Enzyme electrode

[References]

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Olschewski H, Erlenkotter A, Zaborosch C, Chemnitius GC, Enzyme Microb. Technol., 26(7), 537 (2000)
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Yoon KJ, Anal. Sci. Technol., 16, 504 (2003)
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Kwon HS, Jin EH, Yoon KJ, Lee BG, J. Kor. Chem. Soc., 49, 224 (2005)
Yoon KJ, Elastomer., 42, 112 (2007)
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Henle ES, Linn S, J. Biol. Chem., 272, 19095 (1997)
Yoon KJ, J. Kor. Chem. Soc., 52, 197 (2008)
Yoon KJ, J. Kor. Chem. Soc., 48, 654 (2004)
Dı´az AN, Peinado MCR, Minguez MCT, Anal. Chim. Acta., 363, 221 (1998)
Miao Y, Tan SN, Anal. Chim. Acta., 437, 87 (2001)
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[Referenced By]

[1] de Oliveira IRWZ, Vieira IC, Enzyme Microb. Technol., 38(3-4), 449 (2006)

[2] Wang Z, Jin G, J. Biochem. Biophys. Methods., 57, 203 (2003)

[3] Reshetilov AN, Donova MV, Dovbnya DV, Boronin AM, Biosens. Bioelectron., 11, 401 (1996)

[4] Sanz VC, Mena ML, Corte´ s AG, Ya´n.ez-Sedeno P, Pingarro´ n JM, Anal. Chim.Acta., 528, 1 (2005)

[5] Wang X, Zhang H, Wang E, Han Z, Hu C, Mater. Lett., 58, 1661 (2004)

[6] Mailley P, Cummings EA, Mailley S, Cosnier S, Eggins BR, McAdams E, Biochemistry., 63, 291 (2004)

[7] Vostiar I, Tkac J, Strudik E, Gemeiner P, Biochemistry., 56, 113 (2002)

[8] Wang J, Anal. Chim. Acta., 399, 21 (1999)

[9] Wang B, Zhang J, Dong S, Biosens. Bioelectron., 15, 397 (2000)

[10] Chen X, Zhang J, Wang B, Cheng G, Dong S, Anal. Chim. Acta., 434, 255 (2001)

[11] Olschewski H, Erlenkotter A, Zaborosch C, Chemnitius GC, Enzyme Microb. Technol., 26(7), 537 (2000)

[12] Ogon´ czyk D, Tymecki Ł. Wyz˙ kiewicz I, Koncki R, Gła˛b S, Sens. Actuators B., 106, 450 (2005)

[13] Yoon KJ, Anal. Sci. Technol., 16, 504 (2003)

[14] Yoon KJ, Bull. Kor. Chem. Soc., 25, 997 (2004)

[15] Kwon HS, Jin EH, Yoon KJ, Lee BG, J. Kor. Chem. Soc., 49, 224 (2005)

[16] Yoon KJ, Elastomer., 42, 112 (2007)

[17] Lee C, Lee KB, J. Ind. Eng. Chem., 14(4), 473 (2008)

[18] Yoon KJ, Anal. Sci. Technol., 20, 49 (2007)

[19] Choi SY, Yoon KJ, Elastomer., 41, 231 (2006)

[20] Yoon KJ, Bull. Kor. Chem. Soc., 29, 2264 (2008)

[21] Brydson JA, Rubbery Materials and Their Compounds, vol. 149, Elsevier Applied Science, London and New York, England (1988)

[22] Mansouri A, Makris DPM, Kefalas P, J. Pham, Biochem. Anal., 39, 39 (2005)

[23] Graft E, Mahoney JR, Bryant RG, Eaton JW, J. Biol. Chem., 259, 3620 (1984)

[24] Henle ES, Linn S, J. Biol. Chem., 272, 19095 (1997)

[25] Yoon KJ, J. Kor. Chem. Soc., 52, 197 (2008)

[26] Yoon KJ, J. Kor. Chem. Soc., 48, 654 (2004)

[27] Dı´az AN, Peinado MCR, Minguez MCT, Anal. Chim. Acta., 363, 221 (1998)

[28] Miao Y, Tan SN, Anal. Chim. Acta., 437, 87 (2001)

[29] Wang J, Ye H, Jiang Z, Chen N, Huang J, Anal. Chim. Acta., 508, 171 (2004)

[30] Tang B, Wang Y, Spectrochim. Acta Part A., 59, 2867 (2003)