High cell density fermentation of Saccharomyces cerevisiae GS2 for selenium-enriched yeast production

Zheng Wang; Liyang Zhang; Tianwei Tan

Korean Journal of Chemical Engineering, Vol.27, No.6, 1836-1840, November, 2010

DOI10.1007/s11814-010-0300-x Export Citation

High cell density fermentation of Saccharomyces cerevisiae GS2 for selenium-enriched yeast production

Zheng Wang, Liyang Zhang, and Tianwei Tan^†

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. China

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This paper describes a fed-batch fermentation protocol about production of selenium-enriched yeast. Saccharomyces cerevisiae GS2 was selected because of its high tolerance to selenium. The strain GS2 was tested and 122± 0.5 g·l-1 dry cell weight was obtained after 30 h cultivation through feed back control of feed rate of glucose according to the concentration of ethanol and dissolved oxygen. Furthermore, the optimal pattern of Na2SeO3 addition was 9 mg Na2SeO3 against 1 g DCW at late exponential phase. With the combination of glucose feeding and Na2SeO3 addition, the final dry cell biomass reached 102±0.4 g·l-1 and a Se uptake level of 2,020±13 mg·Kg-1 was achieved in a 5 l fermentor after 38 h cultivation.

Keywords:High Cell Density Fermentation;Saccharomyces cerevisiae;Selenium;Yeast

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[2] Clark LC, Combs GF, Turnbull BW, Slate EH, Chalker DK, Chow J, Davis LS, Glover RA, Graham GF, Gross EG, Krongrad A, Lesher JL, Park HK, Sanders BB, Smith CL, Taylor JR, J. Am. Med. Assoc., 276, 1957 (1996)

[3] Finley JW, Ip C, Lisk DJ, Davis CD, Hintze KJ, Whanger PD, J. Agric. Food Chem., 49, 2679 (2001)

[4] Klein EA, Lippman SM, Thompson IM, Goodman PJ, Albanes D, Taylor PR, Coltman C, World J. Urol., 21, 21 (2003)

[5] Bologna R, Indacochea F, Shor-Posner G, Mantero-Atienza E, Grazziutii M, Sotomayor MC, Fletcher M, Cabrejos C, Scott GB, Baum MK, J. Nutr. Immunol., 3, 41 (1994)

[6] Letavayova L, Vlckova V, Brozmanova J, Toxicology, 227, 1 (2006)

[7] Demirci A, Pometto AL, Cox DJ, J. Agric. Food Chem., 47, 2496 (1999)

[8] Demirci A, Pometto III AL, J. Agric. Food Chem., 47, 2491 (2000)

[9] Suhajda A, Hegoczki J, Janzso B, Pais I, Vereczkey G, J. Trace Elem. Med. Bio., 14, 43 (2000)

[10] Gharieb MM, Gadd GM, Mycol. Res., 108, 1415 (2004)

[11] Wang Z, Tan T, Song J, Process Biochem., 42, 108 (2007)

[12] Shang F, Wang Z, Tan TW, Appl. Microbiol. Biotechnol., 77(6), 1233 (2008)

[13] Yin H, Chen Z, Gu Z, Gao H, LWT Food Sci. Technol., 42, 327 (2009)

[14] Tan TW, Zhang M, Gao H, Enzyme Microb. Technol., 33(4), 366 (2003)

[15] Chen J, Zhang SL, Chu J, Zhuang YP, Luo JL, Bai H, Biotechnol. Lett., 26(2), 109 (2004)