화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.19, No.6, 573-581, June, 2011
DOI10.1007/s13233-011-0606-5  Export Citation
Effect of Cross-Linking Spacers on Biocompatibility of Chitosan-Spacer-Poly(ethylene oxide) Hydrogel
Doyeon Kim, Sumi Kim, Seongyeon Jo, Junghoon Woo, and Insup Noh†
  • Dept. of Chemical Engineering, Seoul National University of Science and Technology, Seoul, 139-743, Korea
E-mail:
A cross-linking spacer molecule in biomaterials is an important factor in controlling their biocompatibility. The effects of various cross-linking spacers on the biocompatibility of chitosan-spacer-poly(ethylene oxide) (PEO) hydrogel were evaluated by various cytotoxicity assays and by targeting to specific cell organs. The chitosanspacer-PEO hydrogel was synthesized using a Michael type addition reaction by employing chitosan-acrylate and PEO-thiols. Chitosan-acrylates have been synthesized employing three different cross-linking spacers such as 2-carboxyethyl acrylate, linoleic acid and oleic acid. This study verified the grafting of the spacer molecules to the amine groups of chitosan side chains with FTIR by comparing the peaks of the chitosan-acrylates with those of the unmodified chitosan. After evaluating the cytotoxicity of the chitosan-acrylates in solution with a live a dead assay, the cytotoxicity of the chitosan-spacer-PEO gels was compared with each other by testing them with CCK-8 for their effects on cell proliferation, with a MTT assay for their effects on mitochondria damage, with BrdU assay for their effects on DNA damages and with the neutral red assay for their effects on lysosome damages. The degrees of cytotoxicity of the chitosan-spacer-PEO hydrogels were compared with those of Teflon and Latex, positive and negative control, respectively, using neural cells, such as PC-12 cells. The chitosan-2-carboxyethyl acrylate-PEO hydrogels demonstrated the best cell compatibility among the hydrogels employed in this study.
Keywords:chitosan;cross-linking agents;biocompatibility;hydrogel.
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