A flexible pentacene thin film transistors as disposable DNA hybridization sensor

Jung-Min Kim; Sandeep Kumar Jha; Dong-Hoon Lee; Rohit Chand; Jun-Ho Jeun; Yong-Sang Kim

Journal of Industrial and Engineering Chemistry, Vol.18, No.5, 1642-1646, September, 2012

DOI10.1016/j.jiec.2012.02.026 Export Citation

A flexible pentacene thin film transistors as disposable DNA hybridization sensor

Jung-Min Kim¹, Sandeep Kumar Jha¹, Dong-Hoon Lee¹, Rohit Chand¹, Jun-Ho Jeun¹, and Yong-Sang Kim^{1, 2, †}

¹Department of Nano Science & Engineering, Myongji University, Gyeonggi-do 449-728, Republic of Korea
²Department of Electrical Engineering, Myongji University, Gyeonggi-do 449-728, Republic of Korea

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We fabricated a disposable DNA hybridization sensor using pentacene thin film transistors (TFTs) on flexible substrate. The analytes, 100-mer ss-DNA (poly A and poly T) or 100 bp ds-DNA were immobilized directly on the surface of the pentacene layer, thereby producing a dramatic change in the electrical properties of the devices. The electrical characteristics of devices were studied as a function of DNA immobilization conditions, DNA concentrations and between ss- and ds-DNA. Based on these results, we propose a disposable label-free sensor for DNA hybridization through direct measurement of electrical properties by the immobilization of DNA on flexible pentacene TFTs.

Keywords:Hybridization sensor;Pentacene thin film transistor;Disposable sensor;Label-free

[References]

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Nam JM, Stoeva SI, Mirkin CA, J. Am. Chem. Soc., 126(19), 5932 (2004)
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Marshall A, Hodgson J, Nat. Biotechnol., 16, 27 (1998)
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Kim JM, Jha SK, Chand R, Lee DH, Kim YS, Biosens. Bioelectron., 26, 2264 (2011)

[Referenced By]

[1] Patolsky F, Lichtenstein A, Willner I, Nat. Biotechnol., 19, 253 (2001)

[2] Nam JM, Stoeva SI, Mirkin CA, J. Am. Chem. Soc., 126(19), 5932 (2004)

[3] Ramsay G et al., Nat. Biotechnol., 16, 40 (1998)

[4] Marshall A, Hodgson J, Nat. Biotechnol., 16, 27 (1998)

[5] Pividori MI, Merkoci A, Alegret S, Biosens. Bioelectron., 15, 291 (2000)

[6] Boon EM, Salas JE, Barton JK, Nat. Biotechnol., 20, 282 (2002)

[7] Tang X, Bansaruntip S, Nakayama N, Yenilmez E, Chang YI, Wang Q, Nano Lett., 6, 1632 (2006)

[8] Wang J, Bard A, J. Anal. Chem., 73, 2207 (2001)

[9] Nelson BP, Grimsrud TE, Liles MR, Goodman RM, Corn RM, Anal. Chem., 73, 1 (2001)

[10] Estrale P, Migliorato P, J. Mater. Chem., 17, 219 (2007)

[11] McKendry R, Zhang JY, Arntz Y, Strunz T, Hegner M, Lang HP, Baller MK, Certa U, Meyer E, Guntherodt HJ, Gerber C, PNAS., 99, 9783 (2002)

[12] Yan F, Mok SM, Yu J, Chan HLW, Yang M, Biosens. Bioelectron., 24, 1241 (2009)

[13] Zang QT, Subramanian V, Biosens. Bioelectron., 22, 3182 (2007)

[14] Kim JM, Jha SK, Chand R, Lee DH, Kim YS, Biosens. Bioelectron., 26, 2264 (2011)