실크/PLGA 하이브리드 필름에서 실크가 인간 대동맥 내피세포의 부착과 증식에 미치는 효과

이지혜; 이소진; 김슬지; 김경희; 김영래; 송정은; 이동원; 강길선; Jihye Lee; Sojin Lee; Seulji Kim; Kyounghee Kim; Younglae Kim; Jeongeun Song; Dongwon Lee; Gilson Khang

Polymer(Korea), Vol.37, No.2, 127-134, March, 2013

Export Citation

실크/PLGA 하이브리드 필름에서 실크가 인간 대동맥 내피세포의 부착과 증식에 미치는 효과

Effect of Silk in Silk/PLGA Hybrid Films on Attachment and Proliferation of Human Aortic Endothelial Cells

이지혜, 이소진, 김슬지, 김경희, 김영래, 송정은 , 이동원 ^†, 강길선 ^†

전북대학교 BIN 융합공학과, 고분자 융합소재 연구센터, 고분자·나노공학과

Jihye Lee, Sojin Lee, Seulji Kim, Kyounghee Kim, Younglae Kim, Jeongeun Song , Dongwon Lee^†, and Gilson Khang^†

Dept. of BIN Fusion Tech, Polymer Fusion Research Center & Dept. of Polymer Nano Sci Tech, Chonbuk National University, 567 Baekje-daero, Deokjin, Jeonju 561-756, Korea

E-mail:,

초록

혈관내피세포는 혈관 안쪽을 덮고 있는 편평한 세포층으로, 혈관의 기능과 혈관평활근세포의 증식을 조절한다. 폴리락타이드글리콜라이드 공중합체(PLGA)는 물성이 좋고 분해속도를 조절하기 좋은 생분해성 합성고분자이며, 여러 형태로 제조하기 쉽다. 누에에서 얻은 실크 피브로인은 18가지 아미노산으로 구성되어 있고 세포의 부착과 세포 기능 유지에 중요하며 화장품, 의료분야 등 다양한 분야에서 응용되고 있다. 본 연구에서는 용매 증발법을 이용하여 0, 10, 20, 40 및 80 wt%의 실크를 이용하여 실크/PLGA 하이브리드 필름을 만들었으며, MTT, SEM, ELISA, 면역세포화학염색법을 실시하였다. 실크/PLGA 하이브리드 필름에서 실크 함량에 따른 인간 대동맥 내피세포의 부착과 증식을 측정한 결과, 40 wt%의 실크/PLGA 하이브리드 필름에서 세포의 부착과 증식이 가장 높았으며, 이런 결과들은 실크가 세포의 증식에 좋은 영향을 미치고 실크/PLGA 하이브리드 필름의 표면이 인간 대동맥 내피세포의 성장에 알맞은 환경이라는 것을 확인할 수 있었다.

The vascular endothelial cells are the inner layers of blood vessels. It regulates the function of blood vessels and proliferation of vascular smooth muscle cells. Poly(lactide-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with a well-controlled degradation rate and an acceptable mechanical strength. It can be easily fabricated into many shapes. Silk consists of 18 amino acids. It found important for attaching cells cultured in vitro, and maintaining cell functions. In this study, we fabricated silk/PLGA biomaterial hybrid films of 0, 10, 20, 40 and 80 wt% silk. We performed MTT, SEM, ELISA, and immunocytochemistry analyses. We confirmed the adhesion and the proliferation of HAECs on silk/PLGA according to the content of silk, and 40 wt% silk/PLGA hybrid films have superior adhesion and proliferation properties. These results demonstrate that silk/PLGA hybrid films provide suitable surfaces for HAECs, and there is the effect of silk on cell growth and proliferation.

Keywords:human aortic endothelial cells;PLGA;silk;hybrid film.

[References]

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Langer R, Tirrell DA, Nature., 428, 487 (2004)
Lee JH. Park SJ, Chun HJ, Kim CH, Int. J. Tissue Reg., 1, 1 (2010)
Petit-Zeman S, Nature Biotech., 19, 201 (2001)
Atala A, J. Endourol., 14, 49 (2000)
Zhang N, Ya H, Wen X, Brain Res. Reviews., 49, 48 (2005)
Khang G, Jeon EK, Rhee JM, Lee I, Lee SJ, Lee HB, Macromol. Res., 11(5), 334 (2003)
Wu XS, in Encyclopedic Handbook of Biomaterials and Bioengineering, Part A: Materials, Wise DL, Trantolo DJ, Altobelli DE, Yaszemski MJ, Gresser JD, Schwartz ER, Editors, Marcel Dekker, New York, 1, 1015 (1995)
Shishatskaya EI, Volova TG, Puzyr AP, Mogilnaya OA, Efremov SN, J. Mater. Sci : Mater. Med., 15, 719 (2004)
Volova T, Shishatskaya E, Sevastianov V, Efremov S, Mogilnaya O, Biochem. Eng. J., 16, 125 (2003)
Iwasaki Y, Sawada S, Nakabayashi N, Khang G, Lee HB, Ishihara K, Biomaterials., 20, 2185 (1999)
Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y, J.Biomed. Mater. Res., 29, 1215 (1995)
Altman G, Horan RLH, Moreau J, Martin I, Richmond J, Kaplan DL, Biomaterials., 23, 4131 (2002)
Reneker DH, Chun I, Nanotechnology., 7, 216 (1996)
Lee OJ, Lee JM, Jin HJ, Park CH, Int. J. Tissue Reg., 1, 68 (2010)
Minoura N, Tsukada M, Nagura M, Polymer., 31, 265 (1990)
Minoura N, Aiba S, Higuchi M, Gotoh Y, Tsukada M, Imai Y, Biochem. Biophys. Res. Commun., 208, 511 (1995)
Santin M, Motta A, Freddi G, Cannas M, J. Biomed. Mater.Res., 46, 382 (1999)
Kweon HY, Cho CS, Int. J. Indust. Entomol., 3, 1 (2001)
Panilaitis B, Altman GH, Chen J, Jang HJ, Karageorgiou V, Kaplan DL, Biomaterials., 24, 3079 (2003)
Egan BM, Lu G, Greene EL, PLEFA., 60, 411 (1999)
Bosnjak ZJ, Anesthesiology., 79, 1392 (1993)
Furchgott RF, Circ. Res., 53, 557 (1983)
Dzau VJ, Am. J. Med., 77, 31 (1984)
Steinberg HO, Tarshoby M, Monestel R, Hook G, Cronin J, Johnson A, Bayazeed B, Baron AD, J. Clin. Invest., 100, 1230 (1997)
Kim HL, Yoo H, Park HJ, Kim YG, Lee D, Kang YS, Khang G, Polym.(Korea), 35(1), 7 (2011)
Liu H, Fan H, Wang Y, Toh SL, Goh J, Biomaterials., 29, 662 (2008)
Park JK, NICE., 21, 613 (2003)
Mooney DJ, Cima L, Langer R, Hansen LK, Ingber DE, Vacanti JP, Mater. Res. Soc. Symp. Proc., 252, 345 (1992)
Mikos AG, Temenoff JS, Elec. J. Biotech., 3, 1 (2000)
Jo EH, Kim SJ, Cho SJ, Lee GY, Kim OY, Lee EY, Cho WH, Lee DW, Khang G, Polym.(Korea), 35(4), 289 (2011)
Keller JC, Schneider GB, Stanford CM, Kellogg B, Implant Dentistry., 12, 175 (2003)
Baeuerle PA, Henkel T, Annu. Rev. Immunol., 12, 141 (1994)
Kim NY, Kim HJ, Lee JH, Lee EK, Kang OH, Kwon DY, So HS, Lee KN, Chong MS, Korean J. Oriental Physiology & Pathology., 25, 227 (2011)
Sah MK, Pramanik K, IJESD., 1, 404 (2010)

[Referenced By]

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[1] Park HN, Lee JB, Kwon IK, Int. J. Tissue Reg., 1, 10 (2010)

[2] Langer R, Tirrell DA, Nature., 428, 487 (2004)

[3] Lee JH. Park SJ, Chun HJ, Kim CH, Int. J. Tissue Reg., 1, 1 (2010)

[4] Petit-Zeman S, Nature Biotech., 19, 201 (2001)

[5] Atala A, J. Endourol., 14, 49 (2000)

[6] Zhang N, Ya H, Wen X, Brain Res. Reviews., 49, 48 (2005)

[7] Khang G, Jeon EK, Rhee JM, Lee I, Lee SJ, Lee HB, Macromol. Res., 11(5), 334 (2003)

[8] Wu XS, in Encyclopedic Handbook of Biomaterials and Bioengineering, Part A: Materials, Wise DL, Trantolo DJ, Altobelli DE, Yaszemski MJ, Gresser JD, Schwartz ER, Editors, Marcel Dekker, New York, 1, 1015 (1995)

[9] Shishatskaya EI, Volova TG, Puzyr AP, Mogilnaya OA, Efremov SN, J. Mater. Sci : Mater. Med., 15, 719 (2004)

[10] Volova T, Shishatskaya E, Sevastianov V, Efremov S, Mogilnaya O, Biochem. Eng. J., 16, 125 (2003)

[11] Iwasaki Y, Sawada S, Nakabayashi N, Khang G, Lee HB, Ishihara K, Biomaterials., 20, 2185 (1999)

[12] Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y, J.Biomed. Mater. Res., 29, 1215 (1995)

[13] Altman G, Horan RLH, Moreau J, Martin I, Richmond J, Kaplan DL, Biomaterials., 23, 4131 (2002)

[14] Reneker DH, Chun I, Nanotechnology., 7, 216 (1996)

[15] Lee OJ, Lee JM, Jin HJ, Park CH, Int. J. Tissue Reg., 1, 68 (2010)

[16] Minoura N, Tsukada M, Nagura M, Polymer., 31, 265 (1990)

[17] Minoura N, Aiba S, Higuchi M, Gotoh Y, Tsukada M, Imai Y, Biochem. Biophys. Res. Commun., 208, 511 (1995)

[18] Santin M, Motta A, Freddi G, Cannas M, J. Biomed. Mater.Res., 46, 382 (1999)

[19] Kweon HY, Cho CS, Int. J. Indust. Entomol., 3, 1 (2001)

[20] Panilaitis B, Altman GH, Chen J, Jang HJ, Karageorgiou V, Kaplan DL, Biomaterials., 24, 3079 (2003)

[21] Egan BM, Lu G, Greene EL, PLEFA., 60, 411 (1999)

[22] Bosnjak ZJ, Anesthesiology., 79, 1392 (1993)

[23] Furchgott RF, Circ. Res., 53, 557 (1983)

[24] Dzau VJ, Am. J. Med., 77, 31 (1984)

[25] Steinberg HO, Tarshoby M, Monestel R, Hook G, Cronin J, Johnson A, Bayazeed B, Baron AD, J. Clin. Invest., 100, 1230 (1997)

[26] Kim HL, Yoo H, Park HJ, Kim YG, Lee D, Kang YS, Khang G, Polym.(Korea), 35(1), 7 (2011)

[27] Liu H, Fan H, Wang Y, Toh SL, Goh J, Biomaterials., 29, 662 (2008)

[28] Park JK, NICE., 21, 613 (2003)

[29] Mooney DJ, Cima L, Langer R, Hansen LK, Ingber DE, Vacanti JP, Mater. Res. Soc. Symp. Proc., 252, 345 (1992)

[30] Mikos AG, Temenoff JS, Elec. J. Biotech., 3, 1 (2000)

[31] Jo EH, Kim SJ, Cho SJ, Lee GY, Kim OY, Lee EY, Cho WH, Lee DW, Khang G, Polym.(Korea), 35(4), 289 (2011)

[32] Keller JC, Schneider GB, Stanford CM, Kellogg B, Implant Dentistry., 12, 175 (2003)

[33] Baeuerle PA, Henkel T, Annu. Rev. Immunol., 12, 141 (1994)

[34] Kim NY, Kim HJ, Lee JH, Lee EK, Kang OH, Kwon DY, So HS, Lee KN, Chong MS, Korean J. Oriental Physiology & Pathology., 25, 227 (2011)

[35] Sah MK, Pramanik K, IJESD., 1, 404 (2010)