화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.29, No.2, 176-184, April, 2018
DOI10.14478/ace.2017.1113  Export Citation
금전초 추출물 및 분획물의 항산화 활성 및 세포 보호 효과
Antioxidative and Cellular Protective Effects of Lysimachia christinae Hance Extract and Fractions
김아랑, 정민철1, 정혜인1, 송동기1, 서영빈1, 전영희1, 박소현, 신혁수, 이상래, 박수남†
  • 서울과학기술대학교 정밀화학과
  • 1나노바이오화장품연구실 화장품종합기술연구소
A Rang Kim, Min Chul Jung1, Hye In Jeong1, Dong Gi Song1, Young Bin Seo1, Young Hee Jeon1, So Hyun Park, Hyuk Soo Shin, Sang Lae Lee, and Soo Nam Park†
  • Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneungro, Nowon-gu, Seoul 01811, Korea
  • 1Hansung Science High School, Seoul 03732, Korea
E-mail:
초록
본 논문에서는 금전초로부터 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 제조하였고이들의 항산화 활성, 세포 보호 효과 및 성분 분석에 대한 연구를 수행하였다. 항산화능 평가에서 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획의 라디칼 소거 활성(FSC50)은 각각 146.8, 22.2 및 27.2 μg/mL이었고, 총 항산화능(OSC50)은 29.3, 2.9 및 4.5 μg/mL이었다. 에틸아세테이트 분획의 자유라디칼 소거 활성 및 총 항산화능이 가장 크게 나타났다. 또한 1O2로 유도된 적혈구 광용혈에 대한 세포 보호 효과(τ50)는 금전초 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획 5 μg/mL에서 각각 26.9, 57.5 및 103.9 min을 나타냈다. 특히 아글리콘 분획물의 τ50은 (+)-α-tocopherol (37.7 min)보다 훨씬 큰 세포 보호 효과를 나타내었다. UVB로 유도된 세포 손상에서 에틸아세테이트 분획물은 최대 90.1%까지 세포 생존율을 증가시켰다. 과산화수소로 유도된 세포 손상에서도 에틸아세테이트 분획물은 5-25 μg/mL에서 농도 의존적으로 세포 보호 효과를 나타내었다. 금전초 에틸아세테이트 분획물의 성분 분석을 위해 TLC, HPLC, UV-vis spectrum, LC-MS로 분석한 결과, quercetin, kaempferol 및 그 배당체가 주성분들로 확인되었다. 결론적으로 금전초가 자외선에 노출된 피부를 보호하는 항산화제로서 작용할 수 있고, 기능성 화장품 원료로 응용 가능성이 있음을 시사한다.
In the present study, we investigated the antioxidative properties, cellular protective effects and component analyses of 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Lysimachia christinae Hance (L. christinae Hance). In the evaluation of antioxidative properties, the free radical scavenging activities (FSC50) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction were 146.8, 22.2 and 27.2 μg/mL, respectively and total antioxidant capacities (OSC50) were 29.3, 2.9 and 4.5 μg/mL, respectively. The ethyl acetate fraction showed the highest free radical scavenging activity and total antioxidant capacity. Also, the cellular protective effects (τ50) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction on 1O2 induced photohemolysis of human erythrocytes were 26.9, 57.5 and 103.9 min at 5 μg/mL, respectively. In particular, τ50 of the aglycone fraction exhibited a higher cellular protective effect than that of (+)-α-tocopherol (37.7 min). The cell viability of the ethyl acetate fraction on the UVB-induced cell damage increased up to 90.1%. In addition, the ethyl acetate fraction (5-25 μg/mL) showed cellular protective effects on the H2O2-induced cell damages in a dose-dependent manner. TLC, HPLC, UV-vis spectroscopy and LC-MS were used to analyse components of the ethyl acetate fraction and the main components were quercetin, kaempferol and their glycosides. In conclusion, L. christinae Hance extract/fraction can function as antioxidants to protect the skin exposed to UV radiation and may also be used as a novel functional cosmetic material, for example, an antioxidant against skin photoaging.
Keywords:Lysimachia christinae Hance;reactive oxygen species;antioxidant;component analysis;cellular protective effect
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