In vitro skin permeation and cellular protective effects of flavonoids isolated from Suaeda asparagoides extracts

Soo Nam Park; Sun Young Kim; Gyu Nam Lim; Na Rae Jo; Min Hye Lee

Journal of Industrial and Engineering Chemistry, Vol.18, No.2, 680-683, March, 2012

DOI10.1016/j.jiec.2011.11.126 Export Citation

In vitro skin permeation and cellular protective effects of flavonoids isolated from Suaeda asparagoides extracts

Soo Nam Park^†, Sun Young Kim, Gyu Nam Lim, Na Rae Jo, and Min Hye Lee

Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, 232, Gongreung-ro, Nowon-gu, Seoul 139-743, Republic of Korea

E-mail:

The objective of this study was to compare astragalin (kaempferol-3-O-glucoside) isolated from Suaeda asparagoides extract with kaempferol, an aglycone of astragalin showing the anti-oxidative activity, the cellular protective effect, and skin permeability. Kaempferol was superior to (+)-a-tocopherol and Lascorbic acid known as strong antioxidants in the antioxidative activity by DPPH method and chemiluminescence assay. Astragalin and kaempferol exhibited more prominent cellular protective effect than the lipid peroxidation chain blocker, (+)-a-tocopherol. In this in vitro permeation experiment of astragalin and kaempferol through the ICR mouse skin, kaempferol permeated deeper into the skin than astragalin. These results indicate that kaempferol and astragalin can function as antioxidants in biological systems, particularly skin exposed to solar radiation, and protect cellular membranes against reactive oxygen species.

Keywords:Suaeda asparagoides;Kaempferol;Astragalin;Cellular protective effect

[References]

Foote CS, Photosensitized Oxidation and Singlet Oxygen, 2nd ed., Academic press, New York (1976)
Park SN, Ph.D. Thesis, Seoul National University, Seoul, Korea (1989)
Park SN, J. Korean Cosmet. Sci., 23, 75 (1997)
Park SN, Korean J. Food Sci. Technol., 35, 510 (2003)
Park SN, J. Korean Ind. Eng. Chem., 14(5), 657 (2003)
Vile GF, Tyrrell RM, Free Radic. Biol. Med., 18, 721 (1995)
Scharffetter-Kochanek K, Wlaschek M, Briviba K, Sies H, Febs Lett., 331, 304 (1993)
Wlaschek M, Briviba K, Stricklin GP, Sies H, Scharffetter-Kochanek KK, J. Invest.Dermatol., 104, 194 (1995)
Oikarinen A, Kallioinen M, Photodermatology., 6, 24 (1989)
Kligman LH, UVA-induced biochemical changes in hairless mouse skin collagen: a contrast to F U.V.B. effects, in: Urbach F (Ed.), Biologic Responses to UVA Radiation, Valdenmar, Overland, 209 (1992)
Choi JW, Kim SI, Kim JY, Yang HJ, Lee KH, Park SN, J. Korean Cosmet. Sci., 32, 181 (2006)
Yang HJ, Park SN, J. Korean Cosmet. Sci., 33, 61 (2007)
Kim JY, Park SN, J. Korean Cosmet. Sci., 34, 83 (2008)
Tyrrell RM, Pidoux M, Photochem. Photobiol., 49, 407 (1989)
Foote CS, Photosensitized Oxidation and Singlet Oxygen: Consequences in Biological Systems in Free Radical in Biology, 2nd ed., Academic press, New York (1976)
Havsteen B, Plant Cell Physiol., 28, 1141 (1983)
Husain SR, Gillard J, Cillard P, Phytochemistry., 26, 2489 (1987)
Torel J, Cillard J, Cillard P, Phytochemistry., 25, 383 (1986)
Park JS, Rho HS, Kim DH, Chang IS, J. Agric. Food Chem., 54, 2951 (2006)
Singh R, Singh B, Singh S, Kumar N, Kumar S, Arora S, Toxicol. In Vitro., 22, 1965 (2008)
Luo H, Rankin GO, Li Z, Priest LD, Chen YC, Food Chem., 218, 513 (2011)
Mylonis I, Lakka A, Tsakalof A, Simos G, Biochem. Biophys. Res. Commun., 398(1), 74 (2010)
Jeon SM, Kim SI, Ahn JY, Park SN, J. Korean Cosmet. Sci., 33, 145 (2007)
Jeon SM, Ahn JY, Park SN, J. Korean Cosmet. Sci., 33, 231 (2007)
Yang HJ, Park SN, J. Korean Cosmet. Sci., 34, 157 (2007)
Fujita Y, Uehara L, Morimoto Y, Nakashima M, Hatano T, Okuda T, Yakugaku Zasshi., 108, 129 (1988)
Tsai YH, Lee KF, Huang YB, Huang CT, Wu PC, Int. J. Pharm., 388, 257 (2010)
Heard CM, Johnson S, Moss G, Thomas CP, Int. J. Pharm., 317, 26 (2006)
Batchelder RJ, Calder RJ, Thomas CP, Heard CM, Int. J. Pharm., 283, 45 (2004)
Cho WG, J. Korean Oil Chem. Soc., 24, 410 (2007)

[Referenced By]

[Related Articles]

[1] Foote CS, Photosensitized Oxidation and Singlet Oxygen, 2nd ed., Academic press, New York (1976)

[2] Park SN, Ph.D. Thesis, Seoul National University, Seoul, Korea (1989)

[3] Park SN, J. Korean Cosmet. Sci., 23, 75 (1997)

[4] Park SN, Korean J. Food Sci. Technol., 35, 510 (2003)

[5] Park SN, J. Korean Ind. Eng. Chem., 14(5), 657 (2003)

[6] Vile GF, Tyrrell RM, Free Radic. Biol. Med., 18, 721 (1995)

[7] Scharffetter-Kochanek K, Wlaschek M, Briviba K, Sies H, Febs Lett., 331, 304 (1993)

[8] Wlaschek M, Briviba K, Stricklin GP, Sies H, Scharffetter-Kochanek KK, J. Invest.Dermatol., 104, 194 (1995)

[9] Oikarinen A, Kallioinen M, Photodermatology., 6, 24 (1989)

[10] Kligman LH, UVA-induced biochemical changes in hairless mouse skin collagen: a contrast to F U.V.B. effects, in: Urbach F (Ed.), Biologic Responses to UVA Radiation, Valdenmar, Overland, 209 (1992)

[11] Choi JW, Kim SI, Kim JY, Yang HJ, Lee KH, Park SN, J. Korean Cosmet. Sci., 32, 181 (2006)

[12] Yang HJ, Park SN, J. Korean Cosmet. Sci., 33, 61 (2007)

[13] Kim JY, Park SN, J. Korean Cosmet. Sci., 34, 83 (2008)

[14] Tyrrell RM, Pidoux M, Photochem. Photobiol., 49, 407 (1989)

[15] Foote CS, Photosensitized Oxidation and Singlet Oxygen: Consequences in Biological Systems in Free Radical in Biology, 2nd ed., Academic press, New York (1976)

[16] Havsteen B, Plant Cell Physiol., 28, 1141 (1983)

[17] Husain SR, Gillard J, Cillard P, Phytochemistry., 26, 2489 (1987)

[18] Torel J, Cillard J, Cillard P, Phytochemistry., 25, 383 (1986)

[19] Park JS, Rho HS, Kim DH, Chang IS, J. Agric. Food Chem., 54, 2951 (2006)

[20] Singh R, Singh B, Singh S, Kumar N, Kumar S, Arora S, Toxicol. In Vitro., 22, 1965 (2008)

[21] Luo H, Rankin GO, Li Z, Priest LD, Chen YC, Food Chem., 218, 513 (2011)

[22] Mylonis I, Lakka A, Tsakalof A, Simos G, Biochem. Biophys. Res. Commun., 398(1), 74 (2010)

[23] Jeon SM, Kim SI, Ahn JY, Park SN, J. Korean Cosmet. Sci., 33, 145 (2007)

[24] Jeon SM, Ahn JY, Park SN, J. Korean Cosmet. Sci., 33, 231 (2007)

[25] Yang HJ, Park SN, J. Korean Cosmet. Sci., 34, 157 (2007)

[26] Fujita Y, Uehara L, Morimoto Y, Nakashima M, Hatano T, Okuda T, Yakugaku Zasshi., 108, 129 (1988)

[27] Tsai YH, Lee KF, Huang YB, Huang CT, Wu PC, Int. J. Pharm., 388, 257 (2010)

[28] Heard CM, Johnson S, Moss G, Thomas CP, Int. J. Pharm., 317, 26 (2006)

[29] Batchelder RJ, Calder RJ, Thomas CP, Heard CM, Int. J. Pharm., 283, 45 (2004)

[30] Cho WG, J. Korean Oil Chem. Soc., 24, 410 (2007)