화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.64, 300-310, August, 2018
DOI10.1016/j.jiec.2018.03.028  Export Citation
Dual-functionalized calcium nanocomplexes for transfection of cancerous and stem cells: Low molecular weight polycation-mediated colloidal stability and ATP-mediated endosomal release
Yeon Su Choi1, Kyoungnam Kim1, Kitae Ryu1, Hana Cho1, Yong-Yeon Cho1, Joo Young Lee1, Hye Suk Lee1, Byung Gon Kim2, 3, Soo Chang Song4, 5, and Han Chang Kang1, †
  • 1Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomicsbased Future Pharmacy, College of Pharmacy, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do 14662, Republic of Korea
  • 2Department of Brain Science and Department of Neurology, School of Medicine, Ajou University, 164 Worldcup-ro, Yeongtong-gu, Suwon, Gyeonggi-do 16499, Republic of Korea
  • 3Neuroscience Program, Department of Biomedical Science, Graduate School of Medicine, Ajou University, 164 Worldcup-ro, Yeongtong-gu, Suwon, Gyeonggi-do 16499, Republic of Korea
  • 4Center for Biomaterials, Korea Institute of Science and Technology (KIST), 5 Hwarang-ro 14 gil, Seongbuk-gu, Seoul 02792, Republic of Korea
  • 5Department of Biomolecular Science, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea
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To overcome colloidal instability of calcium phosphate nanoparticles in gene delivery, colloidally stable and endosomolytic Ca2+-based pDNA nanocomplexes (NCs) were designed by a surface coating of biocompatible polycations (PCs; low molecular weight branched polyethyleneimine [bPEI], protamine sulfate and e-polylysine) and the addition of natural and endosomolytic ATP, respectively. Without remarkable cytotoxicity and colloidal instability, Ca2+/ATP-pDNA/bPEI1.8 kDa NCs having [bPEI1.8 kDa] = 3.6 mg showed 5.8-fold and 4.4-fold higher transfection efficiencies than bPEI25 kDa/pDNA NCs in HepG2 cells and dental pulp stem cells, respectively. In conclusion, pH-sensitive endosomolytic ATP and Ca2+-based gene complexes could be potentials as effective and safe gene delivery vectors in various cells.
Keywords:Adenosine triphosphate;Polycation;Calcium ions;Endosomal escape;pH-responsive
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